Q: May I know the exact formulation for antibody NB300-691 (EFEMP1 antibody)? Other than the 0.02% sodium azide, are there other components like buffer salts, BSA, etc? I am primarily concerned with the possible presence of BSA, Tris, glycine, or gelatin.

A: NB300-691 is supplied in Tris/glycine buffer at pH 7.4 containing 0.02% sodium azide as preservative, cryoprotective agents, stabilizing proteins (BSA) and glycerol.

Fibulin-3/EFEMP1 Antibody

Novus Biologicals | Catalog # NB300-691

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all Fibulin 3 Primary Antibodies »

Product Specifications

Immunogen

Synthetic cyclic peptide corresponding to N terminal amino acids 25-42 of Human EFEMP1, peptide was covalently modified. Sequence: TYTQCTDGYEWDPVRQQC

Localization

Secreted

Specificity

The Anti EFEMP1 selective was generated against a conserved sequence near the N terminal region that is unique to EFEMP1 protein only. This detects a strong 62 kDa EFEMP1 protein in human.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Fibulin-3/EFEMP1 Antibody

Western Blot: Fibulin-3/EFEMP1 Antibody [NB300-691]

Western Blot: EFEMP1 Antibody [NB300-691] - 62 kDa EFEMP1from human RPE cells. Antibody diluted 1:500

Immunohistochemistry-Paraffin: Fibulin-3/EFEMP1 Antibody [NB300-691]

Immunohistochemistry-Paraffin: Fibulin-3/EFEMP1 Antibody [NB300-691] - 1:50 dilution, DAB (brown) staining and Hematoxyin QS (blue) counterstain. 40X magnification, FFPE section

Applications for Fibulin-3/EFEMP1 Antibody

Application

Recommended Usage

ELISA

1:10000

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Application Notes

Detects a band of approximately 62 kDa (predicted molecular weight: 60 kDa). Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fibulin 3

This gene spans approximately 18 kb of genomic DNA and consists of 12 exons. Two transcripts with distinct 5' UTR have been described; the resulting proteins have distinct N-terminal amino acid sequences. Translation initiation from internal methionine residues was observed with in vitro translation. A signal peptide sequence is predicted for translation initiation sites 1, 2, and 4. The protein isoforms contain 5 or 6 calcium-binding EGF2 domains and 5 or 6 EGF2 domains. Mutations in this gene cause the retinal disease Malattia Leventinese.

Alternate Names

DHRD, EFEMP1, FBLN3, MLVT

Entrez Gene IDs

2202 (Human); 305604 (Rat)

Gene Symbol

EFEMP1

Additional Fibulin 3 Products

Product Documents for Fibulin-3/EFEMP1 Antibody

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Fibulin-3/EFEMP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Extracellular Matrix Molecules

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