![Western Blot: Fibulin-3/EFEMP1 Antibody [NB300-691]](https://resources.rndsystems.com/images/products/EFEMP1-Antibody-Western-Blot-NB300-691-img0002.jpg "Western Blot: Fibulin-3/EFEMP1 Antibody [NB300-691]")
Loading...
Key Product Details
Species Reactivity
Human, Mouse, Rat, Monkey
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Loading...
Product Specifications
Immunogen
Synthetic cyclic peptide corresponding to N terminal amino acids 25-42 of Human EFEMP1, peptide was covalently modified. Sequence: TYTQCTDGYEWDPVRQQC
Localization
Secreted
Specificity
The Anti EFEMP1 selective was generated against a conserved sequence near the N terminal region that is unique to EFEMP1 protein only. This detects a strong 62 kDa EFEMP1 protein in human.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Fibulin-3/EFEMP1 Antibody (NB300-691) is a polyclonal antibody validated for use in IHC, WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Fibulin-3/EFEMP1 Antibody
Western Blot: Fibulin-3/EFEMP1 Antibody [NB300-691]
Western Blot: EFEMP1 Antibody [NB300-691] - 62 kDa EFEMP1from human RPE cells. Antibody diluted 1:500![Immunohistochemistry-Paraffin: Fibulin-3/EFEMP1 Antibody [NB300-691]](https://resources.rndsystems.com/images/products/Fibulin-3-EFEMP1-Antibody-Immunohistochemistry-Paraffin-NB300-691-img0003.jpg "Immunohistochemistry-Paraffin: Fibulin-3/EFEMP1 Antibody [NB300-691]")
Immunohistochemistry-Paraffin: Fibulin-3/EFEMP1 Antibody [NB300-691]
Immunohistochemistry-Paraffin: Fibulin-3/EFEMP1 Antibody [NB300-691] - 1:50 dilution, DAB (brown) staining and Hematoxyin QS (blue) counterstain. 40X magnification, FFPE sectionApplications for Fibulin-3/EFEMP1 Antibody
Application
Recommended Usage
ELISA
1:10000
Immunohistochemistry-Paraffin
1:100
Immunoprecipitation
1:200
Western Blot
1:500
Application Notes
Detects a band of approximately 62 kDa (predicted molecular weight: 60 kDa). Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Fibulin 3
Alternate Names
DHRD, EFEMP1, FBLN3, MLVT
Gene Symbol
EFEMP1
Additional Fibulin 3 Products
Product Documents for Fibulin-3/EFEMP1 Antibody
Product Specific Notices for Fibulin-3/EFEMP1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for Fibulin-3/EFEMP1 Antibody
There are currently no reviews for this product. Be the first to review Fibulin-3/EFEMP1 Antibody and earn rewards!
Have you used Fibulin-3/EFEMP1 Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Fibulin-3/EFEMP1 Antibody
Showing
1
-
1 of
1 FAQ
Showing All
-
Q: May I know the exact formulation for antibody NB300-691 (EFEMP1 antibody)? Other than the 0.02% sodium azide, are there other components like buffer salts, BSA, etc? I am primarily concerned with the possible presence of BSA, Tris, glycine, or gelatin.
A: NB300-691 is supplied in Tris/glycine buffer at pH 7.4 containing 0.02% sodium azide as preservative, cryoprotective agents, stabilizing proteins (BSA) and glycerol.
Loading...