Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Fibulin 3 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human Fibulin 3. The immunogen is located within amino acids 410 - 460 of Fibulin 3. Amino Acid Squence: TFRIKSGNENGEFYLRQTS

Specificity

At least three isoforms of Fibulin 3 are known to exist. This antibody is predicted to not cross-react with other Fibulin proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Fibulin-3/EFEMP1 Antibody - BSA Free (NBP1-77040) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Fibulin-3/EFEMP1 Antibody - BSA Free

Western Blot: Fibulin-3/EFEMP1 AntibodyBSA Free [NBP1-77040]

Western Blot: Fibulin-3/EFEMP1 AntibodyBSA Free [NBP1-77040]

Western Blot: Fibulin-3/EFEMP1 Antibody [NBP1-77040] - HeLa cell lysate with EFEMP1 antibody at (A) 0.5 and (B) 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: Fibulin-3/EFEMP1 Antibody - BSA Free [NBP1-77040]

Immunocytochemistry/ Immunofluorescence: Fibulin-3/EFEMP1 Antibody - BSA Free [NBP1-77040]

Immunocytochemistry/Immunofluorescence: Fibulin-3/EFEMP1 Antibody [NBP1-77040] - HeLa cells with EFEMP1 antibody at 20 ug/ml.
Fibulin-3/EFEMP1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Fibulin-3/EFEMP1 Antibody - BSA Free [NBP1-77040] -

Immunocytochemistry/ Immunofluorescence: Fibulin-3/EFEMP1 Antibody - BSA Free [NBP1-77040] - Immunofluorescence of Fibulin 3 in HeLa cells with Fibulin 3 antibody at 20 u/mL.

Applications for Fibulin-3/EFEMP1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Western Blot

0.5-1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fibulin 3

Fibulin 3, also known as epidermal growth factor (EGF)-containing fibulin-like extracellular matrix protein 1 (EFEMP1), is a member of the fibulin family of extracellular glycoproteins, a group of proteins that are widely distributed and frequently associated with vascular and elastic tissues. The fibulin proteins typically contain a tandem array of EGF-like repeats and a fibulin-type COOH-terminal module. Aberrant accumulation of Fibulin 3 in the endoplasmic reticulum of retinal pigment epithelial cells has been shown to be associated with inherited forms of macular degeneration, but the loss of Fibulin 3 expression does not lead to macular degeneration but rather the appearance of hernias due to a reduction of elastic fibers of fascial connective tissue. Recent experiments have shown that expression of Fibulin 3 promotes tumor growth and may thus be a therapeutic target. At least three isoforms of Fibulin 3 are known to exist. This antibody is predicted to not cross-react with other Fibulin proteins.

Alternate Names

DHRD, EFEMP1, FBLN3, MLVT

Entrez Gene IDs

2202 (Human); 216616 (Mouse); 305604 (Rat)

Gene Symbol

EFEMP1

UniProt

Q12805 (Human)

Additional Fibulin 3 Products

Product Documents for Fibulin-3/EFEMP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Fibulin-3/EFEMP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Extracellular Matrix Molecules

Citations for Fibulin-3/EFEMP1 Antibody - BSA Free

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Protocols

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FAQs

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