Fibulin-3/EFEMP1 Antibody

Novus Biologicals | Catalog # NBP2-16277

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Fibulin 3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fibulin-3/EFEMP1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Porcine (89%), Bovine (86%).

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Fibulin-3/EFEMP1 Antibody (NBP2-16277) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Fibulin-3/EFEMP1 Antibody

Western Blot: Fibulin-3/EFEMP1 Antibody [NBP2-16277]

Western Blot: Fibulin-3/EFEMP1 Antibody [NBP2-16277]

Western Blot: Fibulin-3/EFEMP1 Antibody [NBP2-16277] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with EFEMP1 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Fibulin-3/EFEMP1 Antibody [NBP2-16277]

Immunocytochemistry/ Immunofluorescence: Fibulin-3/EFEMP1 Antibody [NBP2-16277]

Immunocytochemistry/Immunofluorescence: Fibulin-3/EFEMP1 Antibody [NBP2-16277] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: EFEMP1 protein stained by EFEMP1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Fibulin-3/EFEMP1 Antibody [NBP2-16277]

Immunohistochemistry-Paraffin: Fibulin-3/EFEMP1 Antibody [NBP2-16277]

Immunohistochemistry-Paraffin: Fibulin-3/EFEMP1 Antibody [NBP2-16277] - Human colon carcinoma, using EFEMP1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Fibulin-3/EFEMP1 Antibody

Western Blot: Fibulin-3/EFEMP1 Antibody [NBP2-16277] -

Various whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with EFEMP1 antibody (NBP2-16277) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for Fibulin-3/EFEMP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Fibulin 3

This gene spans approximately 18 kb of genomic DNA and consists of 12 exons. Alternative splice patterns in the 5' UTR result in three transcript variants encoding the same extracellular matrix protein. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. [provided by RefSeq]

Alternate Names

DHRD, EFEMP1, FBLN3, MLVT

Gene Symbol

EFEMP1

UniProt

Q12805 (Human)

Additional Fibulin 3 Products

Product Documents for Fibulin-3/EFEMP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Fibulin-3/EFEMP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Extracellular Matrix Molecules

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Protocols

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FAQs

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