What is Fico/Lite-LymphoH?
Ficoll-based cell separation media is for the isolation of blood cells from human and animal species. Fico/Lite ionic density gradient media are supplied as ready-to-use sterile solutions. These media allow a rapid, simple and reliable separation of cell populations in one centrifugation step. Each lot of product is carefully manufactured and quality controlled to ensure lot-to-lot consistency and reliability. Fico/Lite-LymphoH is suitable for the isolation of viable mononuclear cells from human peripheral blood. The Fico/Lite-LymphoH is supplied with a density of 1.077 0.001 g/mL @ 20 °C and an Osmolality of 290 10 mOs/kg H2O. This cell separation product is an ionic density gradient medium widely used for efficient purification of cells without subjecting the cells to the damaging effects of high osmotic pressure.
For research use only. Not for diagnositic use.
What is Ficoll™?
Ficoll™ is a neutral, highly branched, hydrophilic sucrose polymer that dissolves readily in aqueous solution. Ficoll™ is a registered trademark held by GE Healthcare (Amersham Biosciences), formerly owned by Pharmacia. One of the most common applications for Ficoll™ is as a component of density gradient media for the isolation of eukaryotic cells.
We stored our Fico/Lite-LymphoH incorrectly in the freezer. Can we still use it?
Fico/Lite cell separation media should always be stored at refrigerated temperatures. Upon freezing any Fico/Lite product, gradients can form. Slowly thaw the solution and carefully mix it. If no precipitation is visible, the product should still be suitable for use.
Can Fico/Lite-LymphoH be diluted and used for separation of human platelets?
No, this is not recommended. Fico/Lite-LymphoH is a ready-to-use solution with a density of approximately 1.077. It is also osmotically balanced for use on specific eukaryotic cells by the addition of an appropriate lower molecular weight chemical. Properly balanced cell separation products will usually have a specific, narrow osmolality range that supports the separation of the desired cell type.
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