Fico/Lite-LymphoH™
R&D Systems | Catalog # I40150
Key Product Details
Features
Species
Applications
Product Summary for Fico/Lite-LymphoH™
Product Specifications
Osmolality
Sterility Testing
Formulation, Preparation, and Storage
Shipping
Storage
Product Documents for Fico/Lite-LymphoH™
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Customer Reviews for Fico/Lite-LymphoH™
There are currently no reviews for this product. Be the first to review Fico/Lite-LymphoH™ and earn rewards!
Have you used Fico/Lite-LymphoH™?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- A Guide to the Colony Forming Cell Assay: Methods and Tips
- Cultrex UltiMatrix BME Protocol
- Culture of Mouse Enteric Organoids using Cultrex Basement Membrane Extract
- Culturing BG01V Human Embryonic Stem Cells with Mouse Embryonic Fibroblast (MEF)-Conditioned Media
- Culturing Mouse Cortical Stem Cells: Expansion using the Neurosphere System
- Culturing Rat Cortical Stem Cells: Expansion using the Monolayer System
- Culturing Rat Cortical Stem Cells: Expansion using the Neurosphere System
- Definitive Endoderm Differentiation of BG01V Human Embryonic Stem Cells
- General Support Buffers
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Protocol for CEPT Cocktail Kit
- Protocol for Cell Counting Kit-8
- Protocol for Culturing Embryonic Chick Dorsal Root Ganglion Neurons
- Protocol for Culturing Embryonic Rat Spinal Motor Neurons
- Protocol for Culturing Rat Hippocampal Neurons
- Protocol for Culturing Rat Microglia
- Protocol for MortaLIVE™ Non-Toxic Dye
- Protocol for the Differentiation and Characterization of Human Th1 Cells
- Rat Cortical Stem Cell Culture Expansion Protocol
- The Human Colony Forming Cell (CFC) Assay using Methylcellulose-based Media
- The Mouse Colony Forming Cell (CFC) Assay using Methylcellulose-based Media
- The Mouse Pre-B Colony Forming Cell (CFC) Assay Using Methylcellulose-based Media
- Use of Cultrex Basement Membrane Extract to Improve Take and Growth of Xenografts in Mice
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Fico/Lite-LymphoH™
-
Q: Can Fico/Lite-LymphoH be diluted and used for separation of human platelets?
A: No, this is not recommended. Fico/Lite-LymphoH is a ready-to-use solution with a density of approximately 1.077. It is also osmotically balanced for use on specific eukaryotic cells by the addition of an appropriate lower molecular weight chemical. Properly balanced cell separation products will usually have a specific, narrow osmolality range that supports the separation of the desired cell type.
-
Q: We stored our Fico/Lite-LymphoH incorrectly in the freezer. Can we still use it?
A: Fico/Lite cell separation media should always be stored at refrigerated temperatures. Upon freezing any Fico/Lite product, gradients can form. Slowly thaw the solution and carefully mix it. If no precipitation is visible, the product should still be suitable for use.
-
Q: What is Ficoll™?
A: Ficoll™ is a neutral, highly branched, hydrophilic sucrose polymer that dissolves readily in aqueous solution. Ficoll™ is a registered trademark held by GE Healthcare (Amersham Biosciences), formerly owned by Pharmacia. One of the most common applications for Ficoll™ is as a component of density gradient media for the isolation of eukaryotic cells.
-
Q: Can Fico/Lite-LymphoH be diluted and used for separation of human platelets?
A: No, this is not recommended. Fico/Lite-LymphoH is a ready-to-use solution with a density of approximately 1.077. It is also osmotically balanced for use on specific eukaryotic cells by the addition of an appropriate lower molecular weight chemical. Properly balanced cell separation products will usually have a specific, narrow osmolality range that supports the separation of the desired cell type.
-
Q: We stored our Fico/Lite-LymphoH incorrectly in the freezer. Can we still use it?
A: Fico/Lite cell separation media should always be stored at refrigerated temperatures. Upon freezing any Fico/Lite product, gradients can form. Slowly thaw the solution and carefully mix it. If no precipitation is visible, the product should still be suitable for use.
-
Q: What is Ficoll™?
A: Ficoll™ is a neutral, highly branched, hydrophilic sucrose polymer that dissolves readily in aqueous solution. Ficoll™ is a registered trademark held by GE Healthcare (Amersham Biosciences), formerly owned by Pharmacia. One of the most common applications for Ficoll™ is as a component of density gradient media for the isolation of eukaryotic cells.
-
Q: Can Fico/Lite-LymphoH be diluted and used for separation of human platelets?
A: No, this is not recommended. Fico/Lite-LymphoH is a ready-to-use solution with a density of approximately 1.077. It is also osmotically balanced for use on specific eukaryotic cells by the addition of an appropriate lower molecular weight chemical. Properly balanced cell separation products will usually have a specific, narrow osmolality range that supports the separation of the desired cell type.
-
Q: We stored our Fico/Lite-LymphoH incorrectly in the freezer. Can we still use it?
A: Fico/Lite cell separation media should always be stored at refrigerated temperatures. Upon freezing any Fico/Lite product, gradients can form. Slowly thaw the solution and carefully mix it. If no precipitation is visible, the product should still be suitable for use.
-
Q: What is Ficoll™?
A: Ficoll™ is a neutral, highly branched, hydrophilic sucrose polymer that dissolves readily in aqueous solution. Ficoll™ is a registered trademark held by GE Healthcare (Amersham Biosciences), formerly owned by Pharmacia. One of the most common applications for Ficoll™ is as a component of density gradient media for the isolation of eukaryotic cells.