FIH-1/HIF-1AN Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-54749

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FIH-1/HIF-1AN Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a Recombinant Protein corresponding to amino acids: YLQENIGNGDFSVYSASTHKFLYYDEKKMANFQNFKPRSNREEMKFHEFVEKLQDIQQRGGEERLYLQQTLNDTVGRKI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FIH-1/HIF-1AN Antibody - BSA Free

Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-54749]

Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-54749]

Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-54749] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-54749]

Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-54749]

Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-54749] - Staining of human testis shows strong nuclear and cytoplasmic positivity in seminiferous ducts.
Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-54749]

Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-54749]

Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-54749] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-54749]

Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-54749]

Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-54749] - Staining in human testis and liver tissues using anti-HIF1AN antibody. Corresponding HIF1AN RNA-seq data are presented for the same tissues.

Applications for FIH-1/HIF-1AN Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FIH-1/HIF-1AN

Factor Inhibiting HIF1 (FIH) protein facilitates repression of HIF-1 transcriptional activity by binding to VHL, which acts as a transcriptional corepressor. VHL inhibits HIF-1 alpha transactivation function by recruiting histone deacetylases. Involvement of VHL in association with FIH provides a unifying mechanism for the modulation of HIF-1 alpha protein stabilization and transcriptional activation in response to changes in cellular oxygen concentration.

Long Name

Factor Inhibiting HIF-1

Alternate Names

FIH1, HIF-1AN, HIF1AN, Peptide-aspartate beta-dioxygenase

Gene Symbol

HIF1AN

Additional FIH-1/HIF-1AN Products

Product Documents for FIH-1/HIF-1AN Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for FIH-1/HIF-1AN Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for FIH-1/HIF-1AN Antibody - BSA Free

There are currently no reviews for this product. Be the first to review FIH-1/HIF-1AN Antibody - BSA Free and earn rewards!

Have you used FIH-1/HIF-1AN Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies

Associated Pathways

HIF Repressor Pathways HIF Repressor Pathway Thumbnail