![Immunohistochemistry-Paraffin: FLI1 Antibody [NBP2-16500]](https://resources.rndsystems.com/images/products/FLI1-Antibody-Immunohistochemistry-Paraffin-NBP2-16500-img0002.jpg "Immunohistochemistry-Paraffin: FLI1 Antibody [NBP2-16500]")
Loading...
Key Product Details
Species Reactivity
Validated:
Human, Rat
Predicted:
Bovine (94%), Chicken (91%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human FLI1. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (83%).
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for FLI1 Antibody - BSA Free
Immunohistochemistry-Paraffin: FLI1 Antibody [NBP2-16500]
Immunohistochemistry-Paraffin: FLI1 Antibody [NBP2-16500] - Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using antibody at 1:500 dilution.![Western Blot: FLI1 Antibody [NBP2-16500]](https://resources.rndsystems.com/images/products/FLI1-Antibody-Western-Blot-NBP2-16500-img0010.jpg "Western Blot: FLI1 Antibody [NBP2-16500]")
Western Blot: FLI1 Antibody [NBP2-16500]
Western Blot: FLI1 Antibody [NBP2-16500] - A. 50 ug rat kidney lysate/extract 10 % SDS-PAGE FLI1 antibody dilution: 1:1000![Immunoprecipitation: FLI1 Antibody [NBP2-16500]](https://resources.rndsystems.com/images/products/FLI1-Antibody-Immunoprecipitation-NBP2-16500-img0006.jpg "Immunoprecipitation: FLI1 Antibody [NBP2-16500]")
Immunoprecipitation: FLI1 Antibody [NBP2-16500]
Immunoprecipitation: FLI1 Antibody [NBP2-16500] - IP samples: Raji whole cell extract A. 40 ug Raji whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of FLI1 protein by 4 ug FLI1 antibody (NBP2-16500) 10 % SDS-PAGE The immunoprecipitated FLI1 protein was detected by FLI1 antibody (NBP2-16500) diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Immunocytochemistry/ Immunofluorescence: FLI1 Antibody [NBP2-16500] -
FLI1 antibody detects FLI1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FLI1 protein stained by FLI1 antibody (NBP2-16500) diluted at 1:500.
Blue: Hoechst 33343 staining.
Western Blot: FLI1 Antibody [NBP2-16500] -
Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FLI1 antibody (NBP2-16500) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for FLI1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: FLI1
Long Name
Friend Leukemia Integration 1
Alternate Names
ERGB, SIC-1
Gene Symbol
FLI1
UniProt
Additional FLI1 Products
Product Documents for FLI1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FLI1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for FLI1 Antibody - BSA Free
There are currently no reviews for this product. Be the first to review FLI1 Antibody - BSA Free and earn rewards!
Have you used FLI1 Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...