Flotillin 2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16507

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (99%), Rat (100%), Xenopus (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Flotillin 2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Flotillin 2. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (91%).

Localization

Cell membrane; Peripheral membrane protein; Membrane; caveola; Peripheral membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Flotillin 2 Antibody - BSA Free

Western Blot: Flotillin 2 Antibody [NBP2-16507]

Western Blot: Flotillin 2 Antibody [NBP2-16507]

Western Blot: Flotillin 2 Antibody [NBP2-16507] - Flotillin 2 antibody immunoprecipitates Flotillin 2 protein in IP experiments.IP samples: HCT-116 whole cell extract. A. Control with 4 ug of preimmune Rabbit IgG. B. Immunoprecipitation of Flotillin 2 protein by 4 ug Flotillin 2 antibody. 10 % SDS-PAGE. The immunoprecipitated Flotillin 2 protein was detected by Flotillin 2 antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: Flotillin 2 Antibody [NBP2-16507]

Immunohistochemistry-Paraffin: Flotillin 2 Antibody [NBP2-16507]

Immunohistochemistry-Paraffin: Flotillin 2 Antibody [NBP2-16507] - Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using antibody at 1:100 dilution.
Western Blot: Flotillin 2 Antibody [NBP2-16507]

Western Blot: Flotillin 2 Antibody [NBP2-16507]

Western Blot: Flotillin 2 Antibody [NBP2-16507] - Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: Flotillin 2 Antibody [NBP2-16507]

Western Blot: Flotillin 2 Antibody [NBP2-16507]

Western Blot: Flotillin 2 Antibody [NBP2-16507] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.

Applications for Flotillin 2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Flotillin 2

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling. [provided by RefSeq]

Alternate Names

Epidermal surface antigen, ESA1Membrane component chromosome 17 surface marker 1, ESAchromosome 17, surface marker 1 (35kD protein identified bymonoclonal ECS-1), flotillin 2

Gene Symbol

FLOT2

UniProt

Q14254 (Human)

Additional Flotillin 2 Products

Product Documents for Flotillin 2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Flotillin 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Flotillin 2 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Flotillin 2 Antibody - BSA Free

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  • Q: Can you please tell us if the flotilln 2 antibody NBP2-16507 cross reacts with flotillin 1?

    A: NBP2-16507 does NOT cross-react with flotillin 1.

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