Fluoromount

Novus Biologicals | Catalog # NB900-66726

Novus Biologicals
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Key Product Details

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Product Specifications

Specificity

Aqueous mounting medium for fluorescent staining

Application Notes

This product is compatible with FITC, phycoerythrin, phycocyanin and allophycocyanin. It can also be used with other fluorescent markers such as Texas Red and rhodamine.

Protocol Recommendations
1. Mounting Procedure: Remove red tip from the bottle and cut thetip off with a sharp razor or scissors. This will help achieve asmooth flow of Fluoromount and prevent formation of tinybubbles.
2. Place the bottle upside down in a container before use. This will also help clear Fluoromount of bubbles.
3. Blot excess water from the slide without letting tissue specimendry. Make sure tissue is wet prior to mounting.
4. Apply 2-3 drops of Fluoromount on the tissue section. Gentlyrotate the slide to make a thin. Make sure the tissue is completely covered.
5. Allow Fluoromount to dry for 30-45 minutes at room temperature.
6. Seal coverslip edges with nail polish for long term storage.
7. Removal of Fluoromount: Fluoromount is an aqueous-based mounting medium that can easily be removed by soaking theslides in de-ionized water.
8. Place the slide in a beaker full of de-ionized water on a magnetic stirrer.
9. Leave the slide for few hours or overnight with gentle stirring for complete removal of dry Fluoromount.

Formulation, Preparation, and Storage

Preservative

0.05% Sodium Azide

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at room temperature.

Background: Fluoromount

Fluoromount is an aqueous based mounting medium for cover slipping tissues stained with various fluorescent dyes. This product is compatible with FITC, phycoerythrin, phycocyanin and allophycocyanin. However, it can be used with other fluorescent markers such as Texas Red and rhodamine.

Additional Fluoromount Products

Product Documents for Fluoromount

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Fluoromount

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Customer Reviews for Fluoromount (1)

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  • Fluoromount
    Name: Christopher England
    Verified Customer | Posted 12/05/2016
    Fluoromount was used to mount and coverslip. The solution was works excellent, as there is no photobleaching effect and the sample looks great. This is CD38 staining of lung cancer cells.
    We add a small drop of the solution onto the slide before adding the coverslip.
    Fluoromount NB900-66726

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Protocols

View specific protocols for Fluoromount (NB900-66726):

Mounting Procedure (NB900-66726):
1. Remove the red tip from the bottle and cut the tip off with a sharp razor or scissors. This will help achieve smooth flow of Fluoromount and prevent formation of tiny bubbles.
2. Place the bottle upside down in a container before using. This will help clear Fluoromount of tiny bubbles.
3. Blot excess water from the slide without letting tissue dry. Make sure the tissue is wet prior to mounting.
4. Apply 2-3 drops of Fluoromount on the tissue section and gently apply a cover slip. Allow to dry for 30-45 minutes at room temperature. Seal edges of the coverslip with nail polish for long term storage.

Fluoromount is an aqueous-based mounting medium and can easily be removed by soaking slides in deionized water. Place slide in a beaker full of deionized water on a magnetic stirrer. Leave the slide stirring in water for few hours or overnight for complete removal of dry Fluoromount.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Fluoromount

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  • Q: Does NB900-66726 contains glycerol?

    A: No, Fluoromount does not contain any glycerol.

  • Q: Will NB900-66726 solidify after application?

    A: NB900-66726 is an aqueous mounting, and it won't really harden. For long-term storage, you should seal the edges of the cover slip with clear nail polish.

  • Q: Does NB900-66726 contains glycerol?

    A: No, Fluoromount does not contain any glycerol.

  • Q: Will NB900-66726 solidify after application?

    A: NB900-66726 is an aqueous mounting, and it won't really harden. For long-term storage, you should seal the edges of the cover slip with clear nail polish.

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