Flutax 1

Tocris Bioscience | Catalog # 2226

Green-fluorescent taxol derivative; binds microtubules. Used for: imaging microtubules in live cells. Application: Fluorescent microscopy
Tocris Bioscience

Key Product Details

Description

Green-fluorescent taxol derivative; binds microtubules. Used for: imaging microtubules in live cells. Application: Fluorescent microscopy
View all Microtubule Probes »

Product Description

Key information: Flutax 1 is a green-fluorescent taxol derivative; for microtubules labeling in live cells.

Used for: direct imaging of the microtubule cytoskeleton.

Application: fluorescent microscopy.

Properties and Photophysical Data: Flutax 1 binds microtubules with high affinity (Ka ~ 107M-1). Absorption, fluorescence and fluorescence decay of Flutax 1 in solution are pH sensitive. Excitation and emission maxima (λ) are 495 nm and 520 nm, respectively.

Scientific Data Images for Flutax 1

flutax 1 labeled HeLa cells

Flutax 1 labeled HeLa cells.

Flutax 1 was diluted in HBSS and applied to live HeLa cells at a concentration of 2 μM. HeLa cells were incubated for 1 hour at 37°C. After incubation was complete, Flutax 1 was washed off, and fresh HBSS was applied to the cells. Cells were not fixed because Flutax 1 staining is not retained at all after fixation (photobleach). Staining can be done only on Live cells. Flutax 1 staining in live cells diminishes very rapidly when exposed to light, so the cells should be exposed to light as little as possible to retain good fluorescent signal.

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Product Specifications for Flutax 1

Molecular Weight

1283.30

Formula

C71H66N2O21

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

(2aR,4S,4aS,6R,9S,11S,12S,12aR,12bS)-6,12b-Bis(acetyloxy)-9-[(2R,3S)-3-(benzoylamino)-2-hydroxy-1-oxo-3-phenylpropoxy]-12-(benzoyloxy)-2a,3,4,4a,5,6,9,10,11,12,12a,12b-dodecahydro-11-hydroxy-4a,8,13,13-tetramethyl-5-oxo-7,11-methano-1H-cyclodeca[3,4]benz[1,2-b]oxet-4-yl ester N-[(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)carbonyl]-L-alanine

CAS Number

191930-58-2

PubChem ID

16760423

InChI Key

JMWJJMCMRRQCSY-ADONSEPTSA-N

SMILES

O=C2[C@@]3(C)C([C@@]4(OC(C)=O)[C@H](OC4)C[C@@H]3OC([C@H](C)NC(C7=CC=C(C8=C(C=C%10)C(OC9=C8C=CC(O)=C9)=CC%10=O)C(C(O)=O)=C7)=O)=O)[C@H](OC(C6=CC=CC=C6)=O)[C@@]1(O)[C@](C)(C)C([C@H]2OC(C)=O)=C(C)[C@@H](OC([C@H](O)[C@H]([C@]5=CC=CC=C5)NC(C%11=CC=CC=C%11)=O)=O)C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 128.33 100
Ethanol 128.33 100

Preparing Stock Solutions for Flutax 1

The following data is based on the product molecular weight 1283.30.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 0.78 mL 3.90 mL 7.79 mL
5 mM 0.16 mL 0.78 mL 1.56 mL
10 mM 0.08 mL 0.39 mL 0.78 mL
50 mM 0.02 mL 0.08 mL 0.16 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 10 publications citing the usage of this product.

Product Documents for Flutax 1

Certificate of Analysis

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Product Specific Notices for Flutax 1

For research use only

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