FMO1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16511

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human FMO1. The exact sequence is proprietary.

Reactivity Notes

Mouse (84%), Rat (84%), Dog (88%), Pig (88%), Rabbit (84%), Bovine (85%)

Localization

Microsome membrane; Endoplasmic reticulum membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FMO1 Antibody - BSA Free

Western Blot: FMO1 Antibody [NBP2-16511]

Western Blot: FMO1 Antibody [NBP2-16511]

Western Blot: FMO1 Antibody [NBP2-16511] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE gel, diluted at 1:1000.
FMO1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FMO1 Antibody [NBP2-16511] -

FMO1 antibody [N2C1], Internal detects FMO1 protein at Cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in -20C 100% MeOH for 5 min.
Green: FMO1 protein stained by FMO1 antibody [N2C1], Internal (NBP2-16511) diluted at 1:500.
Blue: Hoechst 33343 staining.

Applications for FMO1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FMO1

Metabolic N-oxidation of the diet-derived amino-trimethylamine (TMA) is mediated by flavin-containing monooxygenase and is subject to an inherited FMO3 polymorphism in man resulting in a small subpopulation with reduced TMA N-oxidation capacity resulting in fish odor syndrome Trimethylaminuria. Three forms of the enzyme, FMO1 found in fetal liver, FMO2 found in adult liver, and FMO3 are encoded by genes clustered in the 1q23-q25 region. Flavin-containing monooxygenases are NADPH-dependent flavoenzymes that catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. [provided by RefSeq]

Alternate Names

dimethylaniline monooxygenase [N-oxide-forming] 1, Dimethylaniline oxidase 1, EC 1.14.13.8, Fetal hepatic flavin-containing monooxygenase 1, flavin containing monooxygenase 1, Flavin-containing monooxygenase 1 (fetal liver), FMO 1

Gene Symbol

FMO1

UniProt

Additional FMO1 Products

Product Documents for FMO1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for FMO1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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