FNTB Antibody (5H4) - BSA Free

Novus Biologicals | Catalog # NBP3-26575

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5H4 expressed in HEK293

Format

BSA Free
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Product Specifications

Immunogen

A synthesized peptide derived from Human FNTB [UniProt P49356]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FNTB Antibody (5H4) - BSA Free

FNTB Antibody (5H4)

Immunoprecipitation: FNTB Antibody (5H4) [NBP3-26575] -

Immunoprecipitation: FNTB Antibody (5H4) [NBP3-26575] - Immunoprecipitating FNTB in K562 whole cell lysate.
Lane 1: Rabbit control IgG instead of NBP3-26575 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26575 (2ug) + K562 whole cell lysate (500ug).
Lane 3: K562 whole cell lysate (10ug).
FNTB Antibody (5H4)

Immunohistochemistry: FNTB Antibody (5H4) [NBP3-26575] -

Immunohistochemistry: FNTB Antibody (5H4) [NBP3-26575] - Image of FNTB Antibody (5H4) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
FNTB Antibody (5H4)

Western Blot: FNTB Antibody (5H4) [NBP3-26575] -

Western Blot: FNTB Antibody (5H4) [NBP3-26575] - Positive Western Blot detected in: 293 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, Rat brain tissue.
All lanes: FNTB Antibody at 1: 2000.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 49, 44 kDa
Observed band size: 49 kDa

Applications for FNTB Antibody (5H4) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: FNTB

Long Name

Protein farnesyltransferase subunit beta

Alternate Names

FTase-beta

Gene Symbol

FNTB

Additional FNTB Products

Product Documents for FNTB Antibody (5H4) - BSA Free

Certificate of Analysis

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Product Specific Notices for FNTB Antibody (5H4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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