FOXB1 Antibody (PCRP-FOXB1-1B7) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-14021
![Protein Array: FOXB1 Antibody (PCRP-FOXB1-1B7) - Azide and BSA Free [NBP3-14021]](https://resources.rndsystems.com/images/products/FOXB1-Antibody-PCRP-FOXB1-1B7-Azide-and-BSA-Free-Protein-Array-NBP3-14021-img0001.jpg "Protein Array: FOXB1 Antibody (PCRP-FOXB1-1B7) - Azide and BSA Free [NBP3-14021]")
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # PCRP-FOXB1-1B7
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant full-length human FOXB1protein (Uniprot: Q99853)
Localization
Nucleus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13926).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for FOXB1 Antibody (PCRP-FOXB1-1B7) - Azide and BSA Free
Protein Array: FOXB1 Antibody (PCRP-FOXB1-1B7) - Azide and BSA Free [NBP3-14021]
Protein Array: FOXB1 Antibody (PCRP-FOXB1-1B7) - Azide and BSA Free [NBP3-14021] - Analysis of Protein Array containing more than 19,000 full-length human proteins using FOXB1 antibody (PCRP-FOXB1-1B7).![FOXB1 Antibody (PCRP-FOXB1-1B7) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: FOXB1 Antibody (PCRP-FOXB1-1B7) - Azide and BSA Free [NBP3-14021] -](https://resources.rndsystems.com/images/products/nbp3-14021_mouse-foxb1-mab-pcrp-foxb1-1b7-azide-and-bsa-free-295202516412238.jpg "Immunocytochemistry/ Immunofluorescence: FOXB1 Antibody (PCRP-FOXB1-1B7) - Azide and BSA Free [NBP3-14021] -")
Immunocytochemistry/ Immunofluorescence: FOXB1 Antibody (PCRP-FOXB1-1B7) - Azide and BSA Free [NBP3-14021] -
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using FOXB1 Antibody (PCRP-FOXB1-1B7) - Azide and BSA Free![FOXB1 Antibody (PCRP-FOXB1-1B7) - Azide and BSA Free Flow Cytometry: FOXB1 Antibody (PCRP-FOXB1-1B7) - Azide and BSA Free [NBP3-14021] -](https://resources.rndsystems.com/images/products/nbp3-14021_mouse-foxb1-mab-pcrp-foxb1-1b7-azide-and-bsa-free-295202516395378.jpg "Flow Cytometry: FOXB1 Antibody (PCRP-FOXB1-1B7) - Azide and BSA Free [NBP3-14021] -")
Flow Cytometry: FOXB1 Antibody (PCRP-FOXB1-1B7) - Azide and BSA Free [NBP3-14021] -
Flow cytometric analysis of PFA-fixed HeLa cells. FOXB1 Antibody (PCRP-FOXB1-1B7) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).Applications for FOXB1 Antibody (PCRP-FOXB1-1B7) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunoprecipitation
1-2 ug per 100-500 ug protein
Application Notes
ELISA: For coating, order this antibody without BSA
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: FOXB1
Alternate Names
FKH5HFKH-5, forkhead box B1, forkhead box protein B1, Transcription factor FKH-5
Gene Symbol
FOXB1
Additional FOXB1 Products
Product Documents for FOXB1 Antibody (PCRP-FOXB1-1B7) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FOXB1 Antibody (PCRP-FOXB1-1B7) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Appropriate Fixation of IHC/ICC Samples
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- ELISA Sample Preparation & Collection Guide
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- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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