Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunocytochemistry

Label

DyLight 594 (Excitation = 593 nm, Emission = 618 nm)

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

E. coli-derived recombinant mouse FoxD3
Met1-Val130
Accession # Q61060

Specificity

Detects mouse FoxD3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FoxD3, rhFoxB2, rhFoxH1, and recombinant mouse FoxC2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications for FoxD3 Antibody [DyLight 594]

Application
Recommended Usage

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product AF5090. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: FoxD3

Embryonic stem cells require the forkhead transcriptional regulator FoxD3 for survival. Following gastrulation, FoxD3 generally gets downregulated, except in the neural crest. A variety of growth factors induce FoxD3 expression, including FGF8 and SNAIL, maintaining the effected cells in an undifferentiated state. Thus defects in FoxD3 induction may cause premature differentiation and/or migration-asociated birth defects.

Long Name

Forkhead Box D3

Alternate Names

CWH3, Genesis, HFH2

Gene Symbol

FOXD3

Additional FoxD3 Products

Product Documents for FoxD3 Antibody [DyLight 594]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for FoxD3 Antibody [DyLight 594]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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