FOXO3 Antibody (CL13589) - Azide and BSA Free

Name: FOXO3 Antibody (CL13589) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-43833
Price: 569 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-43833

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # CL13589

Format

Azide and BSA Free

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Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of O43524, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for FOXO3 Antibody (CL13589) - Azide and BSA Free

Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP3-43833]

Staining of human anaplastic oligodendroglioma shows strong nuclear positivity in neurons.

Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP3-43833]

Staining of human liver shows no nuclear positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP3-43833]

Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP3-43833]

Staining of human gastrointestinal shows weak to moderate nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP3-43833]

Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.

Western Blot: FOXO3 Antibody [NBP3-43833]

Analysis in human cell line SH-SY5Y.

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Applications for FOXO3 Antibody (CL13589) - Azide and BSA Free

Application

Recommended Usage

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml

Application Notes

For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: FoxO3

FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia.

Long Name

Forkhead Box O3

Alternate Names

AF6q21, FKHRL1, FKHRL1P2, FOXO3A

Gene Symbol

FOXO3

Additional FoxO3 Products

Product Documents for FOXO3 Antibody (CL13589) - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for FOXO3 Antibody (CL13589) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.