FoxP1 Antibody (JC12) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80742

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # JC12

Format

Azide and BSA Free
View all FoxP1 Primary Antibodies »

Product Specifications

Immunogen

Human FOXP1 [Uniprot# Q9H334]

Epitope

Within the C-terminus

Localization

Nuclear, Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for FoxP1 Antibody (JC12) - Azide and BSA Free

Western Blot: FoxP1 Antibody (JC12)Azide and BSA Free [NBP2-80742]

Western Blot: FoxP1 Antibody (JC12)Azide and BSA Free [NBP2-80742]

Western Blot: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742] - Western blot analysis of resonicated MCF7 cell lysate (A) and MCF7 cell lysate (B) using FOXP1 antibody at 2 ug/ml. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]

Immunocytochemistry/ Immunofluorescence: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]

Immunocytochemistry/Immunofluorescence: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742] - FOXP1 antibody was tested at 1:25 in MCF-7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]

Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]

Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742] - IHC analysis of formalin-fixed paraffin-embedded tissue section of malignant stromal tumor of the human small bowel using mouse monoclonal FOXP1 antibody (clone JC12) at 5 ug/ml concentration. The carcinoma cells developed an expected and specific strong
Flow Cytometry: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]

Flow Cytometry: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]

Flow Cytometry: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HeLa (B) cells using FOXP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.
Immunohistochemistry: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]

Immunohistochemistry: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]

Immunohistochemistry: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal brain using mouse monoclonal FOXP1 antibody (clone JC12) at 5 ug/ml concentration. The cells in the brain tissue depicted strong specific nuclear along with relatively weak cy
Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]

Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]

Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal breast using FOXP1 antibody (clone JC12) at 5 ug/ml concentration. The breast ductal/acinar epithelial cells and the myoepithelial cells developed a strong nuclear along with
Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]

Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742]

Immunohistochemistry-Paraffin: FoxP1 Antibody (JC12) - Azide and BSA Free [NBP2-80742] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal colon using mouse monoclonal FOXP1 antibody (clone JC12) at 5 ug/ml concentration. Most of the cells depicted an expected strong nuclear with mild cytoplasmic staining. Image

Applications for FoxP1 Antibody (JC12) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 15161711)

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

reported in scientific literature (PMID 17586580)

Western Blot

2ug/ml
Application Notes
Heat induced antigen retrieval with Sodium Citrate buffer pH 6.0 is recommended when using this antibody for IHC-P. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FoxP1

FOXP1 is a member of the Forkhead Box Protein family. These proteins are transcription factors that are characterised by a highly conserved forkhead DNA-binding domain. FOXP1 is a repressor of transcription. It forms homodimers, and also heterodimerises with FOXP2 and FOXP4, to facilitate its binding to DNA for transcriptional regulation. A chromosomal aberration involving FOXP1 is found in acute lymphoblastic leukaemia, and FOXP1 is also implicated in mental retardation with language impairment and autistic features (MRLIAF). Chromosomal translocation of FOXP1 is implicated in mucosa-associated lymphoid tissue (MALT) lymphoma.

Long Name

Forkhead Box P1

Alternate Names

QRF1

Gene Symbol

FOXP1

Additional FoxP1 Products

Product Documents for FoxP1 Antibody (JC12) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for FoxP1 Antibody (JC12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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