FoxP3 Antibody (3G3) - BSA Free

Novus Biologicals | Catalog # NB100-56582

Novus Biologicals

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3G3

Format

BSA Free
View all FoxP3 Primary Antibodies »

Product Specifications

Immunogen

Full-length His-tagged murine FOXP3 was used as the immunogen. The precise epitope is not known, but it has been mapped to the N-terminal portion of the protein (NP_001107849).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FoxP3 Antibody (3G3) - BSA Free

Western Blot: FoxP3 Antibody (3G3)BSA Free [NB100-56582]

Western Blot: FoxP3 Antibody (3G3)BSA Free [NB100-56582]

Western Blot: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the azide free version of NB100-56582. Detection of 1) full-length transfected and 2) mock transfected 293 cell lysate at 2 ug/mL.
Flow Cytometry: FoxP3 Antibody (3G3) - BSA Free [NB100-56582]

Flow Cytometry: FoxP3 Antibody (3G3) - BSA Free [NB100-56582]

Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the Allophycocyanin conjugate of NB100-56582. Staining of FOXP3 in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and this antibody. 1 ug/10^6 cells. Products used: anti-CD4 FITC conjugate.
Flow Cytometry: FoxP3 Antibody (3G3) - BSA Free [NB100-56582]

Flow Cytometry: FoxP3 Antibody (3G3) - BSA Free [NB100-56582]

Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the FITC conjugate of NB100-56582. Staining of FOXP3 in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and this antibody, 5 ug/10^6 cells. Products used: anti-CD4 PE conjugate, anti-CD25 APC conjugate, anti-FOXP3 FITC conjugate.
Flow Cytometry: FoxP3 Antibody (3G3) - BSA Free [NB100-56582]

Flow Cytometry: FoxP3 Antibody (3G3) - BSA Free [NB100-56582]

Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the azide free version of NB100-56582. Staining of 2 x 10^6 mouse lymph node cells using 0.1 ug of PE conjugated antibody.
Flow Cytometry: FoxP3 Antibody (3G3) - BSA Free [NB100-56582]

Flow Cytometry: FoxP3 Antibody (3G3) - BSA Free [NB100-56582]

Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the azide free version of NB100-56582. Staining of human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and NBP2-33297PE (right) at 0.1 ug/10^6 cells. Products used: anti-CD4 FITC conjugate, anti-CD25 APC conjugate.
Flow Cytometry: FoxP3 Antibody (3G3) - BSA Free [NB100-56582]

Flow Cytometry: FoxP3 Antibody (3G3) - BSA Free [NB100-56582]

Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the Allophycocyanin conjugate of NB100-56582. Staining of FOXP3 in mouse splenocytes stimulated with anti-mCD3 and rmIL-2 for 48 hours using isotype control (left) and (right) at 0.1 ug/10^6 cells. Anti-CD25 FITC conjugate this antibody, anti-FOXP3 FITC.
Flow Cytometry: FoxP3 Antibody (3G3) - BSA Free [NB100-56582]

Flow Cytometry: FoxP3 Antibody (3G3) - BSA Free [NB100-56582]

Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the FITC conjugate of NB100-56582. Staining of FOXP3 in mouse splenocytes stimulated with anti-mCD3 and rmIL-2 for 48 hours using isotype control (left) and (right) at 0.5 ug/10^6 cells. Anti-CD25 APC conjugate this antibody, anti-FOXP3 FITC.

Applications for FoxP3 Antibody (3G3) - BSA Free

Application
Recommended Usage

Flow Cytometry

100 ng/ 10^6 cells in 50 uL

Western Blot

2 - 5 ug/mL
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FoxP3

One of the many immunotolerance mechanisms that the immune system has developed to distinguish between self and non-self antigens is regulatory T cells or Tregs. Several elegant experiments using transgenic mice and retrovirus-mediated over expression studies, have led to the identification of FoxP3, a transcription factor, as a specific molecular marker essential for the development and function of Tregs. The primary evidence regarding the involvement of FoxP3 in the development of Tregs was provided in patients suffering from IPEX, a rare and fatal human autoimmune disease. The emergence of Tregs and the role of FoxP3 as a critical player in the negative control of various normal and pathological immune responses holds great promise for the development of novel therapies for autoimmune diseases.

Long Name

Forkhead Box P3

Alternate Names

AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID, foxp3 flow, foxp3 flow cytometry

Entrez Gene IDs

50943 (Human); 20371 (Mouse)

Gene Symbol

FOXP3

UniProt

Q9BZS1

Additional FoxP3 Products

Product Documents for FoxP3 Antibody (3G3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FoxP3 Antibody (3G3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FoxP3 Antibody (3G3) - BSA Free

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FAQs for FoxP3 Antibody (3G3) - BSA Free

Showing  1 - 4 of 4 FAQs Showing All

  • Q: Does FoxP3 antibodies comes in lyophilized form?

    A: FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.

  • Q: Since the protocol to isolate cells from mice seems to be quite long I would like to know if you have experience in staining with Foxp3 and Ror gamma and leaving cells overnight? Would you suggest leaving cells overnight in Fix/Perm or better after having completed the staining? And in which buffer would you leave them then?

    A: Where as FOXP3 and ROR gamma staining can be stopped after the fixation step, we suggest that researchers strictly follow the protocol without variation (even if it takes a long time), when trying for the 1st time. After you establish this for your system, then you can work on optimizing a varied protocol according to your needs and convenience. In this case, after the fix/perm step (Step 13 of NBP2-266911) wash the cells in 1X Staining buffer (2 times) and suspend in Staining buffer (500 ul) overnight. Then, in the morning proceed to Step 14 and continue the protocol.

  • Q: What is the immunogen sequence of this FoxP3 antibody?

    A: The full-length His-tagged murine FOXP3 was used as the immunogen.  The precise epitope is not known, but it has been mapped to the N-terminal portion of the protein.

  • Q: What the theoretical molecular weight for FoxP3 antibodies?

    A: The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.

  • Q: Does FoxP3 antibodies comes in lyophilized form?

    A: FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.

  • Q: Since the protocol to isolate cells from mice seems to be quite long I would like to know if you have experience in staining with Foxp3 and Ror gamma and leaving cells overnight? Would you suggest leaving cells overnight in Fix/Perm or better after having completed the staining? And in which buffer would you leave them then?

    A: Where as FOXP3 and ROR gamma staining can be stopped after the fixation step, we suggest that researchers strictly follow the protocol without variation (even if it takes a long time), when trying for the 1st time. After you establish this for your system, then you can work on optimizing a varied protocol according to your needs and convenience. In this case, after the fix/perm step (Step 13 of NBP2-266911) wash the cells in 1X Staining buffer (2 times) and suspend in Staining buffer (500 ul) overnight. Then, in the morning proceed to Step 14 and continue the protocol.

  • Q: What is the immunogen sequence of this FoxP3 antibody?

    A: The full-length His-tagged murine FOXP3 was used as the immunogen.  The precise epitope is not known, but it has been mapped to the N-terminal portion of the protein.

  • Q: What the theoretical molecular weight for FoxP3 antibodies?

    A: The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.

  • Q: Does FoxP3 antibodies comes in lyophilized form?

    A: FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.

  • Q: Since the protocol to isolate cells from mice seems to be quite long I would like to know if you have experience in staining with Foxp3 and Ror gamma and leaving cells overnight? Would you suggest leaving cells overnight in Fix/Perm or better after having completed the staining? And in which buffer would you leave them then?

    A: Where as FOXP3 and ROR gamma staining can be stopped after the fixation step, we suggest that researchers strictly follow the protocol without variation (even if it takes a long time), when trying for the 1st time. After you establish this for your system, then you can work on optimizing a varied protocol according to your needs and convenience. In this case, after the fix/perm step (Step 13 of NBP2-266911) wash the cells in 1X Staining buffer (2 times) and suspend in Staining buffer (500 ul) overnight. Then, in the morning proceed to Step 14 and continue the protocol.

  • Q: What is the immunogen sequence of this FoxP3 antibody?

    A: The full-length His-tagged murine FOXP3 was used as the immunogen.  The precise epitope is not known, but it has been mapped to the N-terminal portion of the protein.

  • Q: What the theoretical molecular weight for FoxP3 antibodies?

    A: The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.

  • Q: Does FoxP3 antibodies comes in lyophilized form?

    A: FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.

  • Q: Since the protocol to isolate cells from mice seems to be quite long I would like to know if you have experience in staining with Foxp3 and Ror gamma and leaving cells overnight? Would you suggest leaving cells overnight in Fix/Perm or better after having completed the staining? And in which buffer would you leave them then?

    A: Where as FOXP3 and ROR gamma staining can be stopped after the fixation step, we suggest that researchers strictly follow the protocol without variation (even if it takes a long time), when trying for the 1st time. After you establish this for your system, then you can work on optimizing a varied protocol according to your needs and convenience. In this case, after the fix/perm step (Step 13 of NBP2-266911) wash the cells in 1X Staining buffer (2 times) and suspend in Staining buffer (500 ul) overnight. Then, in the morning proceed to Step 14 and continue the protocol.

  • Q: What is the immunogen sequence of this FoxP3 antibody?

    A: The full-length His-tagged murine FOXP3 was used as the immunogen.  The precise epitope is not known, but it has been mapped to the N-terminal portion of the protein.

  • Q: What the theoretical molecular weight for FoxP3 antibodies?

    A: The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.

Showing  1 - 4 of 4 FAQs Showing All
View all FAQs for Antibodies