FoxP3 Antibody (3G3) [PE]

Novus Biologicals | Catalog # NBP2-33297PE

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3G3
View all FoxP3 Primary Antibodies »

Product Specifications

Immunogen

Full-length His-tagged murine FOXP3 was used as the immunogen. The precise epitope is not known, but it has been mapped to the N-terminal portion of the protein (NP_001107849).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for FoxP3 Antibody (3G3) [PE]

Flow Cytometry: FoxP3 Antibody (3G3) [PE] [NBP2-33297PE] - Analysis in 2x10^6 mouse lymph node cells using 0.1 ug of antibody.
Flow Cytometry: FoxP3 Antibody (3G3) [PE] [NBP2-33297PE]

Flow Cytometry: FoxP3 Antibody (3G3) [PE] [NBP2-33297PE]

Flow Cytometry: FOXP3 Antibody (3G3) [NBP2-33297PE] - Analysis in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and NBP2-33297PE (right) at 0.1 ug/10^6 cells. Products used: anti-CD4 FITC conjugate, anti CD25 APC conjugate, anti FOXP3 PE conjugate (NBP2-33297PE).

Applications for FoxP3 Antibody (3G3) [PE]

Application
Recommended Usage

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: FoxP3

One of the many immunotolerance mechanisms that the immune system has developed to distinguish between self and non-self antigens is regulatory T cells or Tregs. Several elegant experiments using transgenic mice and retrovirus-mediated over expression studies, have led to the identification of FoxP3, a transcription factor, as a specific molecular marker essential for the development and function of Tregs. The primary evidence regarding the involvement of FoxP3 in the development of Tregs was provided in patients suffering from IPEX, a rare and fatal human autoimmune disease. The emergence of Tregs and the role of FoxP3 as a critical player in the negative control of various normal and pathological immune responses holds great promise for the development of novel therapies for autoimmune diseases.

Long Name

Forkhead Box P3

Alternate Names

AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID, anti-human foxp3 pe

Gene Symbol

FOXP3

Additional FoxP3 Products

Product Documents for FoxP3 Antibody (3G3) [PE]

Certificate of Analysis

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Product Specific Notices for FoxP3 Antibody (3G3) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FoxP3 Antibody (3G3) [PE]

Showing  1 - 3 of 3 FAQs Showing All

  • Q: Does FoxP3 antibodies comes in lyophilized form?

    A: FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.

  • Q: What is the immunogen sequence of this FoxP3 antibody?

    A: The full-length His-tagged murine FOXP3 was used as the immunogen.  The precise epitope is not known, but it has been mapped to the N-terminal portion of the protein.

  • Q: What the theoretical molecular weight for FoxP3 antibodies?

    A: The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.

  • Q: Does FoxP3 antibodies comes in lyophilized form?

    A: FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.

  • Q: What is the immunogen sequence of this FoxP3 antibody?

    A: The full-length His-tagged murine FOXP3 was used as the immunogen.  The precise epitope is not known, but it has been mapped to the N-terminal portion of the protein.

  • Q: What the theoretical molecular weight for FoxP3 antibodies?

    A: The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.

  • Q: Does FoxP3 antibodies comes in lyophilized form?

    A: FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.

  • Q: What is the immunogen sequence of this FoxP3 antibody?

    A: The full-length His-tagged murine FOXP3 was used as the immunogen.  The precise epitope is not known, but it has been mapped to the N-terminal portion of the protein.

  • Q: What the theoretical molecular weight for FoxP3 antibodies?

    A: The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.

Showing  1 - 3 of 3 FAQs Showing All
View all FAQs for Antibodies