FoxP3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-24397

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Predicted:

Bovine (100%), Canine (100%), Chimpanzee (100%), Equine (100%), Mouse (100%), Opossum (100%), Primate (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FoxP3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to the exon 2 deletion site was used as immunogen.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit FoxP3 Antibody - BSA Free (NBP2-24397) is a polyclonal antibody validated for use in IHC and WB. Anti-FoxP3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for FoxP3 Antibody - BSA Free

Immunohistochemistry-Paraffin: FoxP3 Antibody [NBP2-24397]

Immunohistochemistry-Paraffin: FoxP3 Antibody [NBP2-24397]

Immunohistochemistry-Paraffin: FoxP3 Antibody [NBP2-24397] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using FOXP3 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining was observed. Staining was performed by Histowiz.
Western Blot: FoxP3 Antibody [NBP2-24397]

Western Blot: FoxP3 Antibody [NBP2-24397]

Western Blot: FoxP3 Antibody [NBP2-24397] - Analysis of FOXP3 in (A) FOXP3 transfected and (B) mock transfected cell lysate using NBP2-24397 at 5 ug/ml.

Applications for FoxP3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50

Immunohistochemistry-Frozen

1:10-1:2000

Immunohistochemistry-Paraffin

1:50

Western Blot

2-5 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FoxP3

Forkhead box P3 (FoxP3), aka Scurfin and JM2, is a protein involving in immune system responses and functioning as a transcriptional regulator crucial in the development and inhibitory of regulatory T-Cells (Treg). FoxP3 is a master regulator of the Treg lineage, who requires Ahr and STAT-5 and other additional transcription factors to retains its complete function and differentiation. Scurfin remains steady and constitutively expressed in two conditions: a high level in CD25 + CD4 positive regulatory T cells and a low level in CD4 positive/CD25 negative cells. JM2 is absent in CD4 negative/CD8 positive T cells. FoxP3 defects are related to various cancers and autoimmune diseases, and X-linked syndrome (IPEX), while overexpression may also cause severe immunodeficiency.

Long Name

Forkhead Box P3

Alternate Names

AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID, anti-rat foxp3

Gene Symbol

FOXP3

UniProt

Q9BZS1

Additional FoxP3 Products

Product Documents for FoxP3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for FoxP3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FoxP3 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FoxP3 Antibody - BSA Free

Showing  1 - 3 of 3 FAQs Showing All

    • A: FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.

    • A: A synthetic sequence corresponding to the exon 2 deletion site of FoxP3. The exact sequence is proprietary.

    • A: The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.
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