Frizzled-5 Antibody (2D12) - BSA Free

Novus Biologicals | Catalog # NBP2-37451

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2D12

Format

BSA Free
View all Frizzled-5 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Frizzled-5 (AA:151-217) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

64.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Frizzled-5 Antibody (2D12) - BSA Free

Western Blot: Frizzled-5 Antibody (2D12)BSA Free [NBP2-37451]

Western Blot: Frizzled-5 Antibody (2D12)BSA Free [NBP2-37451]

Western Blot: Frizzled-5 Antibody (2D12) [NBP2-37451] - Analysis using FZD5 mouse mAb against A549 (1), and PC-3 (2) cell lysate.
Flow Cytometry: Frizzled-5 Antibody (2D12) - BSA Free [NBP2-37451]

Flow Cytometry: Frizzled-5 Antibody (2D12) - BSA Free [NBP2-37451]

Flow Cytometry: Frizzled-5 Antibody (2D12) [NBP2-37451] - Analysis of HepG2 cells using FZD5 mouse mAb (green) and negative control (red).
Western Blot: Frizzled-5 Antibody (2D12)BSA Free [NBP2-37451]

Western Blot: Frizzled-5 Antibody (2D12)BSA Free [NBP2-37451]

Western Blot: Frizzled-5 Antibody (2D12) [NBP2-37451] - Analysis using FZD5 mAb against human FZD5 recombinant protein. (Expected MW is 32.5 kDa).
ELISA: Frizzled-5 Antibody (2D12) - BSA Free [NBP2-37451]

ELISA: Frizzled-5 Antibody (2D12) - BSA Free [NBP2-37451]

ELISA: Frizzled-5 Antibody (2D12) [NBP2-37451] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for Frizzled-5 Antibody (2D12) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Frizzled-5

FZD5 is a Frizzled Receptor that mediates Wnt signaling. FZD5 is associated with limb bud and bone marrow stem cell development. FZD5 contributes to the activated state of fibroblast-like synoviocytes from patients with rheumatoid arthritis. Recently, FZD5 has been implicated in melanoma metastasis through the Wnt5a and PKC pathway. FZD5 has been reported to be expressed in fetal kidney, fetal and adult liver, fetal lung, and adult pancreas. ESTs have been isolated from bone, liver/spleen, placenta, and prostate libraries. FZD5 was cloned from a retina cDNA library.

Alternate Names

Frizzled5, Frz-5, FZ-5, FZD5

Gene Symbol

FZD5

Additional Frizzled-5 Products

Product Documents for Frizzled-5 Antibody (2D12) - BSA Free

Certificate of Analysis

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Product Specific Notices for Frizzled-5 Antibody (2D12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Wnt Family
Wnt Receptors

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Protocols

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FAQs

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