Frizzled-5 Antibody (6A3) - Azide and BSA Free

Novus Biologicals | Catalog # H00007855-M01

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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunoprecipitation, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 6A3

Format

Azide and BSA Free
View all Frizzled-5 Primary Antibodies »

Product Specifications

Immunogen

FZD5 (ENSP00000354607, 72 a.a. ~ 161 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PLVEIQCSPDLRFFLCSMYTPICLPDYHKPLPPCRSVCERAKAGCSPLMRQYGFAWPERMSCDRLPVLGRDAEVLCMDYNRSEATTAPPR

Specificity

FZD5 - frizzled homolog 5 (Drosophila)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Frizzled-5 Antibody (6A3) - Azide and BSA Free

Western Blot: Frizzled-5 Antibody (6A3) [H00007855-M01]

Western Blot: Frizzled-5 Antibody (6A3) [H00007855-M01]

Western Blot: Frizzled-5 Antibody (6A3) [H00007855-M01] - Analysis of FZD5 expression in transfected 293T cell line by FZD5 monoclonal antibody (M01), clone 6A3.Lane 1: FZD5 transfected lysate(64.5 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Assay: Frizzled-5 Antibody (6A3) [H00007855-M01]

Proximity Ligation Assay: Frizzled-5 Antibody (6A3) [H00007855-M01]

Proximity Ligation Assay: Frizzled-5 Antibody (6A3) [H00007855-M01] - Analysis of protein-protein interactions between WNT5A and FZD5. HeLa cells were stained with anti-WNT5A rabbit purified polyclonal 1:1200 and anti-FZD5 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunoprecipitation: Frizzled-5 Antibody (6A3) [H00007855-M01]

Immunoprecipitation: Frizzled-5 Antibody (6A3) [H00007855-M01]

Immunoprecipitation: Frizzled-5 Antibody (6A3) [H00007855-M01] - Analysis of FZD5 transfected lysate using anti-FZD5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FZD5 MaxPab rabbit polyclonal antibody.
ELISA: Frizzled-5 Antibody (6A3) [H00007855-M01]

ELISA: Frizzled-5 Antibody (6A3) [H00007855-M01]

ELISA: Frizzled-5 Antibody (6A3) [H00007855-M01] - Detection limit for recombinant GST tagged FZD5 is approximately 0.3ng/ml as a capture antibody.

Applications for Frizzled-5 Antibody (6A3) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Proximity Ligation Assay

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Frizzled-5

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD5 protein is believed to be the receptor for the Wnt5A ligand.

Alternate Names

Frizzled5, Frz-5, FZ-5, FZD5

Entrez Gene IDs

7855 (Human)

Gene Symbol

FZD5

OMIM

601723 (Human)

UniProt

NP_003459

Additional Frizzled-5 Products

Product Documents for Frizzled-5 Antibody (6A3) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Frizzled-5 Antibody (6A3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Wnt Family
Wnt Receptors

Citations for Frizzled-5 Antibody (6A3) - Azide and BSA Free

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