FTO Antibody (5-2H10) - Azide Free

Novus Biologicals | Catalog # NBP2-29512

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Equine (100%), Primate (100%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 5-2H10

Format

Azide Free
View all FTO Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Accession # Q9C0B1

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

Specific for the ~58 kDa FTO protein in Western blots of rat testes lysate. The antibody has also been used for immunocytochemistry with neuronal progenitor cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FTO Antibody (5-2H10) - Azide Free

Western Blot: FTO Antibody (5-2H10) [NBP2-29512]

Western Blot: FTO Antibody (5-2H10) [NBP2-29512]

Western Blot: FTO Antibody (5-2H10) [NBP2-29512] - Western blot of rat testes lysate showing specific immunolabeling of the ~ 58k Fto protein.

Applications for FTO Antibody (5-2H10) - Azide Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
The antibody has also been used for immunocytochemistry with neuronal progenitor cells.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FTO

The FTO gene is the most robust gene for common obesity characterized to date. FTO gene expression has been found to be significantly upregulated in the hypothalamus of rats after food deprivation and strongly negatively correlated with the expression of orexin peptide which is involved in the stimulation of food intake (Fredricksson R et al., 2008). Deletion analysis of FTO gene in mice showed that Fto is functionally involved in the control of both energy intake and energy expenditure (Fischer J et al., 2009)

Long Name

Fat Mass and Obesity Associated

Alternate Names

Alpha-ketoglutarate-dependent dioxygenase FTO

Entrez Gene IDs

79068 (Human)

Gene Symbol

FTO

UniProt

Q9C0B1 (Human)

Additional FTO Products

Product Documents for FTO Antibody (5-2H10) - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FTO Antibody (5-2H10) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Diabetes
Obesity

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FTO Antibody (5-2H10) - Azide Free

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  • Q: Do you have any antibodies against FTO, where you can indicate the sequence of the immunogen?

    A: With regards to other FTO antibodies that we do list the peptide sequence for, the immunogen sequence for catalog number NB110-60935 can be found in the range of amino acids 400-505. Many of the exact immunogen sequences used for our products are proprietary and cannot be disclosed. In these instances we try to provide a reasonable amino acid range to help in your decision.

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