Fumarase Antibody (S08-3F2)

Name: Fumarase Antibody (S08-3F2)
Brand: Novus Biologicals
SKU: NBP3-15029
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-15029

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S08-3F2 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide of human Fumarase (Uniprot#: P07954)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fumarase Antibody (S08-3F2)

Western Blot: Fumarase Antibody (S08-3F2) [NBP3-15029]

Western Blot: Fumarase Antibody (S08-3F2) [NBP3-15029] - Western blot detection of Fumarase in CHO-K1, C6, Jurkat cell lysates using Fumarase Rabbit mAb (1:500 diluted). Predicted band size: 55kDa. Observed band size: 49kDa.

Immunocytochemistry/ Immunofluorescence: Fumarase Antibody (S08-3F2) [NBP3-15029]

Immunocytochemistry/Immunofluorescence: Fumarase Antibody (S08-3F2) [NBP3-15029] - Immunofluorescence of Fumarase (green) in hela using Fumarase Rabbit mAb at dilution 1/50, and DAPI (blue)

Applications for Fumarase Antibody (S08-3F2)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/20

Western Blot

1/1000-1/2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fumarase

Fumarase is encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

Alternate Names

EC 4.2.1.2, Fumarase, fumarate hydratase, fumarate hydratase, mitochondrial, HLRCC, LRCC, MCL, MCUL1

Gene Symbol

Additional Fumarase Products

Product Documents for Fumarase Antibody (S08-3F2)

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Certificate of Analysis

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Product Specific Notices for Fumarase Antibody (S08-3F2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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