Fumarase Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89815

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Fumarase Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Fumarase Antibody - BSA Free

Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-89815]

Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-89815]

Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-89815] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-89815]

Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-89815]

Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-89815] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-89815]

Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-89815]

Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-89815] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Fumarase Antibody - BSA Free Immunohistochemistry-Paraffin: Fumarase Antibody - BSA Free [NBP1-89815]

Immunohistochemistry-Paraffin: Fumarase Antibody - BSA Free [NBP1-89815]

Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Fumarase Antibody - BSA Free Western Blot: Fumarase Antibody - BSA Free [NBP1-89815]

Western Blot: Fumarase Antibody - BSA Free [NBP1-89815]

Analysis in control (vector only transfected HEK293T lysate) and FH over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for Fumarase Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fumarase

Fumarase is encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

Alternate Names

EC 4.2.1.2, Fumarase, fumarate hydratase, fumarate hydratase, mitochondrial, HLRCC, LRCC, MCL, MCUL1

Gene Symbol

FH

Additional Fumarase Products

Product Documents for Fumarase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Fumarase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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