FXR/NR1H4 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-16550

Novus Biologicals
Loading...

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Predicted:

Bovine (94%), Porcine (92%), Rabbit (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Cited:

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
Loading...

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human FXR/NR1H4. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit FXR/NR1H4 Antibody - Azide Free (NBP2-16550) is a polyclonal antibody validated for use in IHC, WB and Simple Western. Anti-FXR/NR1H4 Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for FXR/NR1H4 Antibody - Azide and BSA Free

FXR/NR1H4 Antibody

Immunohistochemistry-Paraffin-NBP2-16550-FXR/NR1H4 Antibody-

Immunohistochemistry-Paraffin-NBP2-16550-FXR/NR1H4 Antibody- Mouse tissues.NR1H4 stained by NR1H4 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
FXR/NR1H4 Antibody

Immunohistochemistry-Paraffin-NBP2-16550-FXR/NR1H4 Antibody-

Immunohistochemistry-Paraffin-NBP2-16550-FXR/NR1H4 Antibody-Rat tissues.NR1H4 stained by NR1H4 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
FXR/NR1H4 Antibody

Western Blot: FXR/NR1H4 Antibody [NBP2-16550] -

Western Blot: FXR/NR1H4 Antibody [NBP2-16550] -Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NR1H4 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
FXR/NR1H4 Antibody

Western Blot: FXR/NR1H4 Antibody [NBP2-16550] -

Western Blot: FXR/NR1H4 Antibody [NBP2-16550] - Biochemical measurements & the mRNA & protein expression of top 5 NRs. A, Results of biochemical parameter measurements. Among the serum indicators, ALT in HFD group higher than it in NC group (P = .0004) & CSP group (P = .0222); the AST in HFD group higher than it in NC group (P = .0006) & CSP group (P = .0149); ALP in HFD group higher than it in NC group (P < .0001) & CSP group (P = .0013), & it lower in NC group than in CSP group (P = .0017); in HFD group, the TC level higher than it in NC group (P < .0001) & CSP group (P = .0003), & in NC group, it lower than it in CSP group (P < .0001); in HFD group, the TG level higher than it in NC group (P = .0011) & CSP group (P = .0476); in HFD group, the GLU level higher than it in NC group (P < .0001) & CSP group (P = .0002); & in HFD group, the HDL‐c level higher than it in NC group (P < .0001) & CSP group (P = .0022), & in NC group, it lower than it in CSP group (P < .0001) (n = 10). Among the liver indicators, in NC group, the TC level lower than it in HFD group (P = .0276) & CSP group (P = .0427); in NC group, the TG level lower than it in HFD group (P = .0427) & CSP group (P = .0448) (n = 6). B, The mRNA expression of top 5 NRs in liver. PPAR gamma in HFD group expressed at a higher level than it in NC group (P = .0072) & CSP group (P = .0489); FXR in HFD group expressed at a lower level than it in NC group (P = .0222) & CSP group (P = .0331); PPAR alpha in HFD group expressed lower than NC group (P = .0143) & CSP group (P = .0082); & RAR alpha in HFD group expressed at a higher level than it in NC group (P = .0001) & CSP group (P = .0012) (n = 10). C, Expression of top 5 NRs in liver measured by automatic WB analysis Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32189432), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for FXR/NR1H4 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000
Application Notes

See Simple Western Antibody Database for Simple Western validation: separated by Size

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FXR/NR1H4

FXR and LXR fall into a category of proteins termed orphan receptors; because of their lack of a defined function, and in the case of LXR, the lack of a defined ligand. FXR has been shown to bind a class of lipid molecules called farnesoids. LXR/RXR heterodimers have highest affinity for DR-4 DNA elements while FXR/RXR heterodimers bind IR-1 elements. Both LXR/RXR and FXR/RXR heterodimers retain their responsiveness to 9-cis retinoic acid. The steroid receptor superfamily acts through direct association with DNA sequences known as hormone response elements (HREs) and bind DNA as either homo- or heterodimers. The promiscuous mediator of heterodimerization, RXR, is the receptor for 9-cis retinoic acid, and dimerizes with VDR, TR, PPAR, as well as several novel receptors including LXR (also referred to as RLD-1) and FXR.

Long Name

Farnesoid X-activated receptor

Alternate Names

BAR, HRR-1, NR1H4, RIP14

Gene Symbol

NR1H4

Additional FXR/NR1H4 Products

Product Documents for FXR/NR1H4 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for FXR/NR1H4 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for FXR/NR1H4 Antibody - Azide and BSA Free

Customer Reviews for FXR/NR1H4 Antibody - Azide and BSA Free

There are currently no reviews for this product. Be the first to review FXR/NR1H4 Antibody - Azide and BSA Free and earn rewards!

Have you used FXR/NR1H4 Antibody - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies
Loading...