G3BP1 Antibody (5A4T0)

Novus Biologicals | Catalog # NBP3-16302

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5A4T0 expressed in HEK293
View all G3BP1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human G3BP1 (Q13283). EVDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGM

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for G3BP1 Antibody (5A4T0)

Western Blot: G3BP1 Antibody (5A4T0) [NBP3-16302]

Western Blot: G3BP1 Antibody (5A4T0) [NBP3-16302]

Western Blot: G3BP1 Antibody (5A4T0) [NBP3-16302] - Western blot analysis of extracts of various cell lines, using G3BP11 Rabbit mAb (NBP3-16302) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody (5A4T0) [NBP3-16302]

Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody (5A4T0) [NBP3-16302]

Immunocytochemistry/Immunofluorescence: G3BP1 Antibody (5A4T0) [NBP3-16302] - Immunofluorescence analysis of NIH-3T3 cells using G3BP11 Rabbit mAb (NBP3-16302) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody (5A4T0) [NBP3-16302]

Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody (5A4T0) [NBP3-16302]

Immunocytochemistry/Immunofluorescence: G3BP1 Antibody (5A4T0) [NBP3-16302] - Immunofluorescence analysis of HeLa cells using G3BP11 Rabbit mAb (NBP3-16302) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation: G3BP1 Antibody (5A4T0) [NBP3-16302]

Immunoprecipitation: G3BP1 Antibody (5A4T0) [NBP3-16302]

Immunoprecipitation: G3BP1 Antibody (5A4T0) [NBP3-16302] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug G3BP11 antibody (NBP3-16302). Western blot was performed from the immunoprecipitate using G3BP11 antibody (NBP3-16302) at a dilition of 1:1000.
G3BP1 Antibody (5A4T0)

Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody (5A4T0) [NBP3-16302] -

Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody (5A4T0) [NBP3-16302] - Confocal imaging of HeLa cells (treated with sodium arsenite) and HeLa cells (untreated) using G3BP1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 100x.
G3BP1 Antibody (5A4T0)

Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody (5A4T0) [NBP3-16302] -

Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody (5A4T0) [NBP3-16302] - Confocal imaging of HeLa cells using G3BP1 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
G3BP1 Antibody (5A4T0)

Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody (5A4T0) [NBP3-16302] -

Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody (5A4T0) [NBP3-16302] - Confocal imaging of PC-12 cells (treated with sodium arsenite) and PC-12 cells (untreated) using G3BP1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 100x.

Applications for G3BP1 Antibody (5A4T0)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: G3BP1

G3BP1 encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Alternate Names

ATP-dependent DNA helicase VIII, EC 3.6.1, EC 3.6.4.12, EC 3.6.4.13, G3BP-1, G3BPRas-GTPase-activating protein SH3-domain-binding protein, GAP binding protein, GAP SH3 domain-binding protein 1, GTPase activating protein (SH3 domain) binding protein 1, hDH VIII, HDH-VIII, MGC111040, ras GTPase-activating protein-binding protein 1, RasGAP-associated endoribonuclease G3BP

Gene Symbol

G3BP1

Additional G3BP1 Products

Product Documents for G3BP1 Antibody (5A4T0)

Certificate of Analysis

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Product Specific Notices for G3BP1 Antibody (5A4T0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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