GAD2/GAD65 Antibody

Novus Biologicals | Catalog # NBP1-33284

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Predicted:

Bovine (93%), Canine (96%), Porcine (95%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all GAD2/GAD65 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GAD2/GAD65. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (86%).

Localization

Cytoplasm, cytosol, Cytoplasmic vesicle, Cell junction, synapse, presynaptic cell membrane, Golgi apparatus membrane

Marker

GABAergic Neuronal Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GAD2/GAD65 Antibody

Western Blot: GAD2/GAD65 Antibody [NBP1-33284]

Western Blot: GAD2/GAD65 Antibody [NBP1-33284]

Western Blot: GAD2/GAD65 Antibody [NBP1-33284] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-33284]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-33284]

Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-33284] - RT2 xenograft. GAD65 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunocytochemistry/ Immunofluorescence: GAD2/GAD65 Antibody [NBP1-33284]

Immunocytochemistry/ Immunofluorescence: GAD2/GAD65 Antibody [NBP1-33284]

Immunocytochemistry/Immunofluorescence: GAD2/GAD65 Antibody [NBP1-33284] - Paraformaldehyde-fixed HeLa, using GAD65 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at 1:2500.
GAD2/GAD65 Antibody

Immunocytochemistry/ Immunofluorescence: GAD2/GAD65 Antibody [NBP1-33284] -

GAD65 antibody detects GAD65 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAD65 stained by GAD65 antibody (NBP1-33284) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for GAD2/GAD65 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 2 reviews rated 5 using NBP1-33284 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GAD2/GAD65

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. [provided by RefSeq]

Long Name

Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa)

Alternate Names

GAD65

Entrez Gene IDs

2572 (Human)

Gene Symbol

GAD2

UniProt

Q05329

Additional GAD2/GAD65 Products

Product Documents for GAD2/GAD65 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GAD2/GAD65 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for GAD2/GAD65 Antibody

Customer Reviews for GAD2/GAD65 Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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  • Name: YONGJIE LAI
    Application: Western Blot
    Sample Tested: mouse brain lysate
    Species: Mouse
    Verified Customer | Posted 10/01/2014
  • Name: YONGJIE LAI
    Application: Immunofluorescence
    Sample Tested: pCMV-GAD65 plasmid transfected HEK293T cells
    Species: Human
    Verified Customer | Posted 10/01/2014

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Protocols

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FAQs

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