GADD34 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-03703

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all GADD34 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 375-674 of human GADD34 (NP_055145.3). ASSSTPATGVFLKSWVYQPGEDTEEEEDEDSDTGSAEDEREAETSASTPPASAFLKAWVYRPGEDTEEEEDEDVDSEDKEDDSEAALGEAESDPHPSHPDQRAHFRGWGYRPGKETEEEEAAEDWGEAEPCPFRVAIYVPGEKPPPPWAPPRLPLRLQRRLKRPETPTHDPDPETPLKARKVRFSEKVTVHFLAVWAGPAQAARQGPWEQLARDRSRFARRITQAQEELSPCLTPAARARAWARLRNPPLAPIPALTQTLPSSSVPSSPVQTTPLSQAVATPSRSSAAAAAALDLSGRRG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GADD34 Antibody - Azide and BSA Free

Western Blot: GADD34 AntibodyAzide and BSA Free [NBP3-03703]

Western Blot: GADD34 AntibodyAzide and BSA Free [NBP3-03703]

Western Blot: GADD34 Antibody [NBP3-03703] - Analysis of extracts of various cell lines, using GADD34 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
GADD34 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GADD34 Antibody - Azide and BSA Free [NBP3-03703] -

Immunocytochemistry/ Immunofluorescence: GADD34 Antibody - Azide and BSA Free [NBP3-03703] - Immunofluorescence analysis of H9C2 cells using GADD34 Rabbit pAb (A16260) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
GADD34 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GADD34 Antibody - Azide and BSA Free [NBP3-03703] -

Immunocytochemistry/ Immunofluorescence: GADD34 Antibody - Azide and BSA Free [NBP3-03703] - Immunofluorescence analysis of L929 cells using GADD34 Rabbit pAb (A16260) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for GADD34 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GADD34

GADD34 is a phosphatase whose expression is increased by exposure of cells to DNA damage or stress induced growth arrest. Induction is correlated with apoptosis following exposure to ionizing radiation.

Long Name

Protein phosphatase 1 regulatory subunit 15A

Alternate Names

PPP1R15A

Gene Symbol

PPP1R15A

Additional GADD34 Products

Product Documents for GADD34 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GADD34 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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FAQs

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