Galectin-1 Antibody (1E8-1B2) - Azide and BSA Free

Novus Biologicals | Catalog # H00003956-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Lambda Clone # 1E8-1B2

Format

Azide and BSA Free
View all Galectin-1 Primary Antibodies »

Product Specifications

Immunogen

LGALS1 (AAH01693, 1 a.a. ~ 135 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD

Specificity

LGALS1 - lectin, galactoside-binding, soluble, 1 (galectin 1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Lambda

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Galectin-1 Antibody (1E8-1B2) - Azide and BSA Free

Western Blot: Galectin-1 Antibody (1E8-1B2) [H00003956-M01]

Western Blot: Galectin-1 Antibody (1E8-1B2) [H00003956-M01]

Western Blot: Galectin-1 Antibody (1E8-1B2) [H00003956-M01] - Analysis of LGALS1 expression in transfected 293T cell line by LGALS1 monoclonal antibody (M01), clone 1E8-1B2.Lane 1: LGALS1 transfected lysate(14.7 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Galectin-1 Antibody (1E8-1B2) [H00003956-M01]

Immunocytochemistry/ Immunofluorescence: Galectin-1 Antibody (1E8-1B2) [H00003956-M01]

Immunocytochemistry/Immunofluorescence: Galectin-1 Antibody (1E8-1B2) [H00003956-M01] - Analysis of monoclonal antibody to LGALS1 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Galectin-1 Antibody (1E8-1B2) [H00003956-M01]

Western Blot: Galectin-1 Antibody (1E8-1B2) [H00003956-M01]

Western Blot: Galectin-1 Antibody (1E8-1B2) [H00003956-M01] - LGALS1 monoclonal antibody (M01), clone 1E8-1B2 Analysis of LGALS1 expression in HepG2.
Immunoprecipitation: Galectin-1 Antibody (1E8-1B2) [H00003956-M01]

Immunoprecipitation: Galectin-1 Antibody (1E8-1B2) [H00003956-M01]

Immunoprecipitation: Galectin-1 Antibody (1E8-1B2) [H00003956-M01] - Analysis of LGALS1 transfected lysate using anti-LGALS1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LGALS1 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: Galectin-1 Antibody (1E8-1B2) [H00003956-M01] - Detection limit for recombinant GST tagged LGALS1 is 1 ng/ml as a capture antibody.
Sandwich ELISA: Galectin-1 Antibody (1E8-1B2) [H00003956-M01] - Detection limit for recombinant GST tagged LGALS1 is 0.03 ng/ml as a capture antibody.

Applications for Galectin-1 Antibody (1E8-1B2) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Galectin-1

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS1 may act as an autocrine negative growth factor that regulates cell proliferation.

Alternate Names

BHL, Galaptin, Galectin1, GBP, L-14, LGALS1

Entrez Gene IDs

3956 (Human)

Gene Symbol

LGALS1

OMIM

150570 (Human)

UniProt

AAH01693 (Human)

Additional Galectin-1 Products

Product Documents for Galectin-1 Antibody (1E8-1B2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Galectin-1 Antibody (1E8-1B2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Galectin-1 Antibody (1E8-1B2) - Azide and BSA Free

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Protocols

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FAQs

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