Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-79855
Recombinant Monoclonal Antibody
![Western Blot: Galectin-1 Antibody (GAL1/2499R)Azide and BSA Free [NBP2-79855]](https://resources.rndsystems.com/images/products/Galectin-1-Antibody-GAL1-2499R-Azide-and-BSA-Free-Western-Blot-NBP2-79855-img0004.jpg "Western Blot: Galectin-1 Antibody (GAL1/2499R)Azide and BSA Free [NBP2-79855]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Protein Array
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # GAL1/2499R
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant fragment (around aa 12-108) of human Galectin-1 protein (exact sequence is proprietary) (Uniprot: P09382)
Localization
Secreted (extracellular matrix)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79721).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free
Western Blot: Galectin-1 Antibody (GAL1/2499R)Azide and BSA Free [NBP2-79855]
Western Blot: Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free [NBP2-79855] - Western Blot Analysis of HeLa cell lysate using Galectin-1 antibody (GAL1/2499R).![Immunohistochemistry-Paraffin: Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free [NBP2-79855]](https://resources.rndsystems.com/images/products/Galectin-1-Antibody-GAL1-2499R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79855-img0002.jpg "Immunohistochemistry-Paraffin: Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free [NBP2-79855]")
Immunohistochemistry-Paraffin: Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free [NBP2-79855]
Immunohistochemistry-Paraffin: Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free [NBP2-79855] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Galectin-1 Antibody (GAL1/2499R).![Western Blot: Galectin-1 Antibody (GAL1/2499R)Azide and BSA Free [NBP2-79855]](https://resources.rndsystems.com/images/products/Galectin-1-Antibody-GAL1-2499R-Azide-and-BSA-Free-Western-Blot-NBP2-79855-img0003.jpg "Western Blot: Galectin-1 Antibody (GAL1/2499R)Azide and BSA Free [NBP2-79855]")
Western Blot: Galectin-1 Antibody (GAL1/2499R)Azide and BSA Free [NBP2-79855]
Western Blot: Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free [NBP2-79855] - Western Blot Analysis of JEG-3 and K562 cell lysate using Galectin-1 antibody (GAL1/2499R).![Protein Array: Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free [NBP2-79855]](https://resources.rndsystems.com/images/products/Galectin-1-Antibody-GAL1-2499R-Azide-and-BSA-Free-Protein-Array-NBP2-79855-img0001.jpg "Protein Array: Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free [NBP2-79855]")
Protein Array: Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free [NBP2-79855]
Protein Array: Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free [NBP2-79855] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Galectin-1 Antibody (GAL1/2499R) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination withApplications for Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free
Application
Recommended Usage
ELISA
2-4 ug/ml
Immunohistochemistry
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Galectin-1
Alternate Names
BHL, Galaptin, Galectin1, GBP, L-14, LGALS1
Gene Symbol
LGALS1
Additional Galectin-1 Products
Product Documents for Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Galectin-1 Antibody (GAL1/2499R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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