Galectin-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16581

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Galectin-1 Primary Antibodies »

Product Specifications

Immunogen

Full length human Galectin-1 Recombinant protein.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (82%), Porcine (85%), Bovine (86%).

Localization

Secreted; extracellular space; extracellular matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Knockout (KO) Validated Rabbit Galectin-1 Antibody - BSA Free (NBP2-16581) is a polyclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Galectin-1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Galectin-1 Antibody [NBP2-16581]

Immunocytochemistry/ Immunofluorescence: Galectin-1 Antibody [NBP2-16581]

Immunocytochemistry/Immunofluorescence: Galectin-1 Antibody [NBP2-16581] - A431.
Galectin-1 Antibody

Western Blot: Galectin-1 Antibody [NBP2-16581] -

Western Blot: Galectin-1 Antibody [NBP2-16581] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Galectin-1 Antibody - BSA Free

Western Blot: Galectin-1 Antibody [NBP2-16581] -

Wild-type (WT) and Galectin 1 knockout (KO) 293T cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (NBP2-16581) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Galectin-1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Galectin-1 Antibody [NBP2-16581] -

Galectin 1 antibody detects Galectin 1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Galectin 1 protein stained by Galectin 1 antibody (NBP2-16581) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Galectin-1 Antibody - BSA Free

Western Blot: Galectin-1 Antibody [NBP2-16581] -

Sample (30 ug of whole cell lysate)
A: BCL-1
15% SDS PAGE
NBP2-16581 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Galectin-1 Antibody - BSA Free

Western Blot: Galectin-1 Antibody [NBP2-16581] -

Galectin 1 antibody detects Galectin 1 protein by western blot analysis.
A. 30 ug PC-12 whole cell lysate/extract
B. 30 ug Rat2 whole cell lysate/extract
15% SDS-PAGE
Galectin 1 antibody (NBP2-16581) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Galectin-1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Galectin-1 Antibody [NBP2-16581] -

Galectin 1 antibody detects Galectin 1 protein at cytoplasm by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Galectin 1 protein stained by Galectin 1 antibody (NBP2-16581) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Galectin-1 Antibody - BSA Free

Immunoprecipitation: Galectin-1 Antibody [NBP2-16581] -

Galectin 1 antibody immunoprecipitates Galectin 1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 ug whole cell lysate/extract of Galectin 1 antibody expressing HeLa cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of Galectin 1 by 2.5 ug of Galectin 1 antibody (NBP2-16581) 15% SDS-PAGE The immunoprecipitated Galectin 1 protein was detected by Galectin 1 antibody (NBP2-16581) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Galectin-1 Antibody - BSA Free

Western Blot: Galectin-1 Antibody [NBP2-16581] -

Sample (30 ug of whole cell lysate)
A: NCI-H929
15% SDS PAGE
NBP2-16581 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Galectin-1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Galectin-1

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq]

Alternate Names

BHL, Galaptin, Galectin1, GBP, L-14, LGALS1

Gene Symbol

LGALS1

UniProt

P09382

Additional Galectin-1 Products

Product Documents for Galectin-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Galectin-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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