Galectin-2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-89690
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
Immunohistochemistry, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LEVKNMDMKPGSTLKITGSIADGTDGFVINLGQGTDKLNLHFNPRFSESTIVCNSLDGSNWGQEQREDHLCFSPGSEVKFTVTFESDKFKVKLPDGHELTFPNRLGHSHLSYLSVRGGFNMSSFKL
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Galectin-2 Antibody - BSA Free
Immunohistochemistry: Galectin-2 Antibody [NBP1-89690]
Galectin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89690-img0013.jpgImmunohistochemistry-Paraffin: Galectin-2 Antibody [NBP1-89690]
Immunohistochemistry-Paraffin: Galectin-2 Antibody [NBP1-89690] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.Immunohistochemistry-Paraffin: Galectin-2 Antibody [NBP1-89690]
Immunohistochemistry-Paraffin: Galectin-2 Antibody [NBP1-89690] - Staining of human lymph node shows strong cytoplasmic positivity in non - germinal center cells.Immunohistochemistry-Paraffin: Galectin-2 Antibody [NBP1-89690]
Immunohistochemistry-Paraffin: Galectin-2 Antibody [NBP1-89690] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.Immunohistochemistry-Paraffin: Galectin-2 Antibody [NBP1-89690]
Immunohistochemistry-Paraffin: Galectin-2 Antibody [NBP1-89690] - Staining of human cerebral cortex shows no positivity in neurons.Immunohistochemistry: Galectin-2 Antibody [NBP1-89690]
Galectin-2-Antibody-Immunohistochemistry-NBP1-89690-img0012.jpgImmunohistochemistry: Galectin-2 Antibody [NBP1-89690] -
Immunohistochemistry: Galectin-2 Antibody [NBP1-89690] - Galectin-2 expression in the maternal decidua of control & GDM placentas. Box & scatter plots (A) showing the IRS for galectin-2 expression in decidua to be significantly higher in GDM placentas (p = 0.001). See Figure 1 for a detailed box plot explanation. Pictures show representative slides for immunohistochemical staining of galectin-2, in the maternal decidua of the control (B) & the GDM (C) placentas. The scale bar equals 200 μm in full size images & 50µm in inserts. Black rectangles indicate the section seen at a higher magnification (minuscular letters). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32244351), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunocytochemistry/ Immunofluorescence: Galectin-2 Antibody [NBP1-89690] -
Immunocytochemistry/ Immunofluorescence: Galectin-2 Antibody [NBP1-89690] - Double immunofluorescence phenotyping of villus cells. Nuclei are stained blue using DAPI. Galectin-2, bound by Cy-3-labled secondary antibody, is stained red. CD31, bound by Cy-2-labeled secondary antibody is stained green, marking fetal endothelial cells. Arrows heads indicate the merging expression of CD31 & galectin-2, visible as yellow. The scale bar equals 1 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32244351), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunohistochemistry: Galectin-2 Antibody [NBP1-89690] -
Immunohistochemistry: Galectin-2 Antibody [NBP1-89690] - Immunohistochemistry control staining. Colon tissue was used as positive (A, a) as well as negative (B, b) control, for anti-galectin-2-antibody. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32244351), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunocytochemistry/ Immunofluorescence: Galectin-2 Antibody [NBP1-89690] -
Immunocytochemistry/ Immunofluorescence: Galectin-2 Antibody [NBP1-89690] - Double immunofluorescence phenotyping of decidual cell. Nuclei are stained blue using DAPI. Galectin-2, bound by Cy-3-labled secondary antibody, is stained red. CK7, bound by Cy-2-labeled secondary antibody is stained green, marking the extra-villous trophoblast (EVT) cells. Arrows heads indicate the merging expression of CK7 & galectin-2 visible as yellow. The scale bar equals 1 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32244351), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Galectin-2 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Galectin-2
Alternate Names
GAL2, Galectin2, LGALS2
Gene Symbol
LGALS2
Additional Galectin-2 Products
Product Documents for Galectin-2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Galectin-2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Galectin-2 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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