Galectin-3 Antibody (OTI1C7) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-71124
![Western Blot: Galectin-3 Antibody (OTI1C7)Azide and BSA Free [NBP2-71124]](https://beta-resources.rndsystems.com/images/products/Galectin-3-Antibody-OTI1C7-Azide-and-BSA-Free-Western-Blot-NBP2-71124-img0001.jpg "Western Blot: Galectin-3 Antibody (OTI1C7)Azide and BSA Free [NBP2-71124]")
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG Clone # OTI1C7
Format
Azide and BSA Free
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Product Specifications
Immunogen
Full length human recombinant protein of human LGALS3 (NP_002297) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG
Description
Novus Biologicals Mouse Galectin-3 Antibody (OTI1C7) - Azide and BSA Free (NBP2-45618) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Galectin-3 Antibody (OTI1C7) - Azide and BSA Free
Western Blot: Galectin-3 Antibody (OTI1C7)Azide and BSA Free [NBP2-71124]
Western Blot: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Galectin-3.![Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]](https://beta-resources.rndsystems.com/images/products/Galectin-3-Antibody-OTI1C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71124-img0021.jpg "Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]")
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]](https://beta-resources.rndsystems.com/images/products/Galectin-3-Antibody-OTI1C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71124-img0002.jpg "Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]")
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]](https://beta-resources.rndsystems.com/images/products/Galectin-3-Antibody-OTI1C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71124-img0003.jpg "Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]")
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]](https://beta-resources.rndsystems.com/images/products/Galectin-3-Antibody-OTI1C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71124-img0004.jpg "Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]")
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]](https://beta-resources.rndsystems.com/images/products/Galectin-3-Antibody-OTI1C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71124-img0005.jpg "Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]")
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]](https://beta-resources.rndsystems.com/images/products/Galectin-3-Antibody-OTI1C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71124-img0006.jpg "Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]")
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]](https://beta-resources.rndsystems.com/images/products/Galectin-3-Antibody-OTI1C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71124-img0007.jpg "Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]")
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]](https://beta-resources.rndsystems.com/images/products/Galectin-3-Antibody-OTI1C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71124-img0008.jpg "Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]")
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]](https://beta-resources.rndsystems.com/images/products/Galectin-3-Antibody-OTI1C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71124-img0009.jpg "Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]")
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]](https://beta-resources.rndsystems.com/images/products/Galectin-3-Antibody-OTI1C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71124-img0010.jpg "Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]")
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]](https://beta-resources.rndsystems.com/images/products/Galectin-3-Antibody-OTI1C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71124-img0011.jpg "Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]")
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]](https://beta-resources.rndsystems.com/images/products/Galectin-3-Antibody-OTI1C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71124-img0012.jpg "Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]")
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]](https://beta-resources.rndsystems.com/images/products/Galectin-3-Antibody-OTI1C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71124-img0013.jpg "Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]")
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]](https://beta-resources.rndsystems.com/images/products/Galectin-3-Antibody-OTI1C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71124-img0014.jpg "Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]")
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]](https://beta-resources.rndsystems.com/images/products/Galectin-3-Antibody-OTI1C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71124-img0015.jpg "Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]")
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]](https://beta-resources.rndsystems.com/images/products/Galectin-3-Antibody-OTI1C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71124-img0016.jpg "Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]")
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]](https://beta-resources.rndsystems.com/images/products/Galectin-3-Antibody-OTI1C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71124-img0017.jpg "Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]")
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]](https://beta-resources.rndsystems.com/images/products/Galectin-3-Antibody-OTI1C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71124-img0018.jpg "Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]")
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]](https://beta-resources.rndsystems.com/images/products/Galectin-3-Antibody-OTI1C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71124-img0019.jpg "Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]")
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]](https://beta-resources.rndsystems.com/images/products/Galectin-3-Antibody-OTI1C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71124-img0020.jpg "Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]")
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]](https://beta-resources.rndsystems.com/images/products/Galectin-3-Antibody-OTI1C7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71124-img0022.jpg "Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]")
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124]
Immunohistochemistry: Galectin-3 Antibody (OTI1C7) - Azide and BSA Free [NBP2-71124] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)Applications for Galectin-3 Antibody (OTI1C7) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: Galectin-3
Alternate Names
AGE-R3, CBP35, GAL3, Galectin3, L29, LGALS3, Mac-2
Gene Symbol
LGALS3
Additional Galectin-3 Products
Product Documents for Galectin-3 Antibody (OTI1C7) - Azide and BSA Free
Product Specific Notices for Galectin-3 Antibody (OTI1C7) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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