gamma Adducin Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-12551

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all gamma Adducin Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human gamma Adducin fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for gamma Adducin Antibody - BSA Free

Immunoprecipitation: gamma Adducin Antibody [NBP3-12551]

Immunoprecipitation: gamma Adducin Antibody [NBP3-12551]

Immunoprecipitation: gamma Adducin Antibody [NBP3-12551] - Lane A:0.5 mg HT-29 Whole Cell Lysate4 uL anti-ADD3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ADD3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 81 kDaObserved band size :95 kDa
Western Blot: gamma Adducin Antibody [NBP3-12551]

Western Blot: gamma Adducin Antibody [NBP3-12551]

Western Blot: gamma Adducin Antibody [NBP3-12551] - Lane A: HT-29 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:81 kDaObserved band size:95 kDa
gamma Adducin Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: gamma Adducin Antibody [NBP3-12551] -

Immunocytochemistry/Immunofluorescence: gamma Adducin Antibody [NBP3-12551] - Staining of ADD3 in HEK293 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with NBP3-12551 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and cell membrane.

Applications for gamma Adducin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

1-5uL/mg of uLysate

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: gamma Adducin

Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta and gamma). Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. The cortical actin cytoskeletal network is lost during apoptosis and adducins are central in the cortical actin network organization. Adducin alpha is a cytoskeletal protein involved with sodium-pump activity in the renal tubule and is associated with hypertension. The expression of Adducin alpha and Adducin gamma is ubiquitous in contrast to the restricted expression of Adducin beta. Adducin beta is expressed at high levels in brain and hematopoietic tissues, such as bone marrow in humans, and spleen in mice.

Alternate Names

ADDLAdducin-like protein 70, adducin 3 (gamma), gamma-adducin

Gene Symbol

ADD3

Additional gamma Adducin Products

Product Documents for gamma Adducin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for gamma Adducin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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