gamma-Synuclein Antibody (4A0F4)

Name: gamma-Synuclein Antibody (4A0F4)
Brand: Novus Biologicals
SKU: NBP3-16144
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16144

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4A0F4 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 48-127 of human gamma-Synuclein (O76070). VVQSVTSVAEKTKEQANAVSEAVVSSVNTVATKTVEEAENIAVTSGVVRKEDLRPSAPQQEGEASKEKEEVAEEAQSGGD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for gamma-Synuclein Antibody (4A0F4)

Western Blot: gamma-Synuclein Antibody (4A0F4) [NBP3-16144]

Western Blot: gamma-Synuclein Antibody (4A0F4) [NBP3-16144] - Western blot analysis of extracts of various cell lines, using gamma-Synuclein Rabbit mAb (NBP3-16144) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Immunohistochemistry-Paraffin: gamma-Synuclein Antibody (4A0F4) [NBP3-16144]

Immunohistochemistry-Paraffin: gamma-Synuclein Antibody (4A0F4) [NBP3-16144] - Immunohistochemistry of paraffin-embedded rat brain using gamma-Synuclein Rabbit mAb (NBP3-16144) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for gamma-Synuclein Antibody (4A0F4)

Application

Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: gamma-Synuclein

Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases. May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway. May be a centrosome-associated protein. Cytoplasm; perinuclear region. Centrosome. Spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle. Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung. PTM: Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases. Brain iron accumulation type 1 (NBIA1, also called Hallervorden-Spatz syndrome), a rare neuroaxonal dystrophy, is histologically characterized by axonal spheroids, iron deposition, Lewy body (LB)-like intraneuronal inclusions, glial inclusions and neurofibrillary tangles. SNCG is found in spheroids but not in inclusions. Belongs to the synuclein family.

Alternate Names

BCSG1, PRSN, SNCG, Synoretin, Synuclein-gamma

Gene Symbol

SNCG

Additional gamma-Synuclein Products

Product Documents for gamma-Synuclein Antibody (4A0F4)

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Certificate of Analysis

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Product Specific Notices for gamma-Synuclein Antibody (4A0F4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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