gamma Tubulin Antibody (4511) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43585

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Drosophila

Cited:

Human

Predicted:

Bovine (100%), Canine (100%), Chimpanzee (100%), Rhesus Macaque (100%), Xenopus (99%), Zebrafish (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 4511

Format

Azide and BSA Free
View all gamma Tubulin Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human gamma Tubulin. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (99%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for gamma Tubulin Antibody (4511) - Azide and BSA Free

Western Blot: gamma Tubulin Antibody (4511) [NBP2-43585]

Western Blot: gamma Tubulin Antibody (4511) [NBP2-43585]

Western Blot: gamma Tubulin Antibody (4511) [NBP2-43585] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat-2 whole cell lysate/extract 10 % SDS-PAGE gamma Tubulin antibody dilution: 1:500.
Western Blot: gamma Tubulin Antibody (4511) [NBP2-43585]

Western Blot: gamma Tubulin Antibody (4511) [NBP2-43585]

Western Blot: gamma Tubulin Antibody (4511) [NBP2-43585] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug C8D30 whole cell lysate/extract C. 30 ug NIH-3T3 whole cell lysate/extract D. 30 ug BCL-1 whole cell lysate/extract E. 30 ug C2C12 whole cell lysate/extract 10 % SDS-PAGE gamma Tubulin antibody dilution: 1:500
gamma Tubulin Antibody (4511) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody (4511) [NBP2-43585] -

TUBA1B antibody detects TUBG1 protein at Cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20C 100% methanol for 5 min.
Green: TUBG1 protein stained by TUBA1B antibody (NBP2-43585) diluted at 1:500.
Blue: Hoechst 33342 staining.
gamma Tubulin Antibody (4511) - Azide and BSA Free

Western Blot: gamma Tubulin Antibody (4511) [NBP2-43585] -

gamma Tubulin antibody [GT4511] detects gamma Tubulin protein by Western blot analysis.
A. 30 ug Jurkat whole cell lysate/extract
B. 30 ug Raji whole cell lysate/extract
C. 30 ug NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
gamma Tubulin antibody [GT4511] (NBP2-43585) dilution: 1:1000
gamma Tubulin Antibody (4511) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody (4511) [NBP2-43585] -

gamma Tubulin antibody [GT4511] detects gamma Tubulin protein at centrosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: gamma Tubulin stained by gamma Tubulin antibody [GT4511] (NBP2-43585) diluted at 1:500.
Red: alpha Tubulin, stained by alpha Tubulin antibody diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for gamma Tubulin Antibody (4511) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

Assay dependent

Western Blot

1:500-1:3000
Application Notes
IHC Assay dependent

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: gamma Tubulin

The gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The g-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing g-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.

Long Name

Tubulin gamma-1 chain

Alternate Names

Gamma-1-tubulin, GCP-1, TUBG, TUBG1

Gene Symbol

TUBG1

Additional gamma Tubulin Products

Product Documents for gamma Tubulin Antibody (4511) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for gamma Tubulin Antibody (4511) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for gamma Tubulin Antibody (4511) - Azide and BSA Free

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Protocols

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