gamma Tubulin Antibody (TU-32) - BSA Free
Novus Biologicals | Catalog # NB110-90616
Key Product Details
Species Reactivity
Validated:
Human, Animal, Plant, Protozoa
Cited:
Human
Applications
Validated:
Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # TU-32
Format
BSA Free
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Product Specifications
Immunogen
human gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449
Epitope
The epitope was located in the aminoacid sequence PDYISW (aa441-446 in human), which is identical for gamma-tubulin 1 and gamma-tubulin 2.
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 29212260).
Marker
Centrosome Marker
Specificity
The TU-32 recognizes an epitope (amino acids 434-449 in human) within C-terminus of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for gamma Tubulin Antibody (TU-32) - BSA Free
Western Blot: gamma Tubulin Antibody (TU-32)BSA Free [NB110-90616]
gamma-Tubulin-Antibody-TU-32-Western-Blot-NB110-90616-img0004.jpgWestern Blot: gamma Tubulin Antibody (TU-32)BSA Free [NB110-90616]
Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Analysis of gamma-Tubulin in porcine brain lysate using NBP1-90616.Western Blot: gamma Tubulin Antibody (TU-32)BSA Free [NB110-90616]
Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Differential reactivity of monoclonal antibodies to gamma-tubulin with human gamma-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human gamma-tubulin 1 (gamma-Tb1) or gamma-tubulin 2 (gamma-Tb2), probed with Abs to gamma-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of gamma-Tb1 or gamma-Tb2 probed with Abs to gamma-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.Western Blot: gamma Tubulin Antibody (TU-32) - BSA Free [NB110-90616] -
Western Blot: gamma Tubulin Antibody (TU-32) - BSA Free [NB110-90616] - Comparative mitochondrial abundance as measured by citrate synthase activity, abundance of VDAC1 & cytochrome oxidase subunit 4(A) Citrate synthase enzyme activity in the H1299 & H1299r cells. (B) Citrate synthase enzyme activity in the P31 & P31r cells. Data are expressed as mean ± SEM for three separate experiments. Statistical analysis was carried out using the student t-test. *** = p< 0.001, ** = p<0.01. For immunoblots, whole cell lysates were prepared from confluent cultures of H1299, H1299r, P31 & P31r cells. Proteins (90 μg) were resolved in 10% SDS-PAGE gels & transferred to a PVDF membrane. Blots were probed for either VDAC1, cytochrome c oxidase subunit 4 or the loading control gamma -tubulin. (C) Representative blot of VDAC1 protein expression from three independent experiments. (D) Representative blot of cytochrome c oxidase subunit 4 protein expression from three independent experiments. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.21885), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for gamma Tubulin Antibody (TU-32) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Western Blot
1 ug/ml
Application Notes
Immunocytochemistry: methanol/acetone fixation required
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Phosphate buffered saline (PBS), pH 7.4
Format
BSA Free
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: gamma Tubulin
Long Name
Tubulin gamma-1 chain
Alternate Names
Gamma-1-tubulin, GCP-1, TUBG, TUBG1
Gene Symbol
TUBG1
UniProt
Additional gamma Tubulin Products
Product Documents for gamma Tubulin Antibody (TU-32) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for gamma Tubulin Antibody (TU-32) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for gamma Tubulin Antibody (TU-32) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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