gamma Tubulin Antibody (TU-32) [PE]

Novus Biologicals | Catalog # NB110-90616PE

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Animal, Plant, Protozoa

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # TU-32
View all gamma Tubulin Primary Antibodies »

Product Specifications

Immunogen

human gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449

Epitope

The epitope was located in the aminoacid sequence PDYISW (aa441-446 in human), which is identical for gamma-tubulin 1 and gamma-tubulin 2.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 29212260).

Marker

Centrosome Marker

Specificity

The TU-32 recognizes an epitope (amino acids 434-449 in human) within C-terminus of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for gamma Tubulin Antibody (TU-32) [PE]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: gamma Tubulin

The gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The g-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing g-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.

Long Name

Tubulin gamma-1 chain

Alternate Names

Gamma-1-tubulin, GCP-1, TUBG, TUBG1

Gene Symbol

TUBG1

Additional gamma Tubulin Products

Product Documents for gamma Tubulin Antibody (TU-32) [PE]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for gamma Tubulin Antibody (TU-32) [PE]

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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