GAP-43 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-81934

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GAP-43 Primary Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized non-phosphopeptide derived from human GAP-43 around amino acids 38~43 (Q-A-S-F-R).

Marker

Neuronal Marker

Specificity

Detects endogenous levels of total GAP-43 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GAP-43 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody [NB100-81934] -

Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody [NB100-81934] - Staining of methanol-fixed Hela cells using NB100-81934
GAP-43 Antibody - BSA Free

Immunohistochemistry-Paraffin: GAP-43 Antibody [NB100-81934] -

Immunohistochemistry-Paraffin: GAP-43 Antibody [NB100-81934] - Analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81934(left) or the same antibody preincubated with blocking peptide (right).
GAP-43 Antibody - BSA Free

Western Blot: GAP-43 Antibody [NB100-81934] -

Western Blot: GAP-43 Antibody [NB100-81934] - Analysis of extract from mouse brain tissue, using NB100-81934 incubated with blocking peptide.

Applications for GAP-43 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAP-43

GAP43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration. GAP43 is also a neuronal growth cone protein thought to be involved in pathfinding.

Long Name

Growth Associated Protein 43

Alternate Names

B-50, Basp2, GAP43, Neuromodulin

Gene Symbol

GAP43

UniProt

P17677 (Human)

Additional GAP-43 Products

Product Documents for GAP-43 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GAP-43 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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