GAPDH-2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-31925

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GAPDH-2 Primary Antibodies »

Product Specifications

Immunogen

This GAPDH-2 antibody was developed against a recombinant protein corresponding to amino acids: KYDSTHGRYKGSVEFRNGQLVVDNHEISVYQCKEPKQIPWRAVGSPYVVESTGVYLSIQAASDHISAGAQRVVIS

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24598113)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GAPDH-2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GAPDH-2 Antibody [NBP2-31925]

Immunocytochemistry/ Immunofluorescence: GAPDH-2 Antibody [NBP2-31925]

Immunocytochemistry/Immunofluorescence: GAPDH-2 Antibody [NBP2-31925] - Staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925]

Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925]

Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925] - Staining in human testis and kidney tissues using anti-GAPDHS antibody. Corresponding GAPDHS RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925]

Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925]

Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925] - Staining of human kidney shows low expression as expected.
Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925]

Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925]

Immunohistochemistry-Paraffin: GAPDH-2 Antibody [NBP2-31925] - Staining of human testis shows high expression.

Applications for GAPDH-2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAPDH-2

GAPDHS encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility.

Long Name

Glyceraldehyde-3-phosphate Dehydrogenase 2

Alternate Names

GAPD2, GAPDH2, GAPDHS, HSD-35

Gene Symbol

GAPDHS

UniProt

O14556 (Human)

Additional GAPDH-2 Products

Product Documents for GAPDH-2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GAPDH-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GAPDH-2 Antibody - BSA Free

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Protocols

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FAQs

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