Gasdermin D Antibody

Novus Biologicals | Catalog # NBP2-80427

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Recombinant protein within human Gasdermin D aa 100-300. (SwissProt: P57764 Human; SwissProt: Q9D8T2 Mouse; SwissProt: Q9D8T2 Mouse)

Localization

Cytosol. Secreted. Plasma membrane.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Gasdermin D Antibody

Western Blot: GSDMDC1 Antibody [NBP2-80427]

Western Blot: GSDMDC1 Antibody [NBP2-80427]

Western Blot: GSDMDC1 Antibody [NBP2-80427] - Analysis of Gasdermin D (N terminal) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SiHa cell lysateLane 2: human kidney tissue lysate
Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody [NBP2-80427]

Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody [NBP2-80427]

Immunocytochemistry/Immunofluorescence: GSDMDC1 Antibody [NBP2-80427] - Staining of Gasdermin D in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-37, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427]

Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427]

Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427] - Analysis in Mouse colon tissue using GSDMDC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with
Flow Cytometry: GSDMDC1 Antibody [NBP2-80427]

Flow Cytometry: GSDMDC1 Antibody [NBP2-80427]

Flow Cytometry: GSDMDC1 Antibody [NBP2-80427] - Analysis of GSDMDC1 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).
Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427]

Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427]

Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427] - Analysis in rat stomach tissue using GSDMDC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with
Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427]

Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427]

Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427] - Analysis in human tonsil tissue using GSDMDC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed wit
Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427]

Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427]

Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427] - Analysis in human prostate carcinoma tissue using GSDMDC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature
Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427]

Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427]

Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427] - Analysis in human esophagus tissue using GSDMDC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed
Gasdermin D Antibody

Gasdermin D Antibody

Western blot analysis of Gasdermin D (N terminal) on THP-1 cell lysates with Gasdermin D Antibody (NBP2-80427) at 1/1,000 dilution.Lysates/proteins at 20 ug/Lane.Predicted band size: 53 kDaObserved band size: 53/35 kDaExposure time: 25 seconds; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for Gasdermin D Antibody

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000

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Advanced Features

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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH7.4), 0.2% BSA, 50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Gasdermin D

Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]

Alternate Names

DF5L, DFNA5L, gasdermin domain containing 1, Gasdermin domain-containing protein 1, gasdermin-D, GSDMDC1

Gene Symbol

GSDMD

Additional Gasdermin D Products

Product Documents for Gasdermin D Antibody

Certificate of Analysis

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Product Specific Notices for Gasdermin D Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Gasdermin D Antibody

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Protocols

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