GAT-1/SLC6A1 Antibody (HL3475)

Novus Biologicals | Catalog # NBP3-48818

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL3475
View all GAT-1/SLC6A1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of mouse GAT-1/SLC6A1. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit GAT-1/SLC6A1 Antibody (HL3475) (NBP3-48818) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for GAT-1/SLC6A1 Antibody (HL3475)

GAT-1/SLC6A1 Antibody (HL3475)

Immunohistochemistry-Paraffin: GAT-1/SLC6A1 Antibody (HL3475) [NBP3-48818] -

GAT-1/SLC6A1 antibody [HL3475] detects GAT-1/SLC6A1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat eye.
Green: GAT-1/SLC6A1 stained by GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GAT-1/SLC6A1 Antibody (HL3475)

Immunohistochemistry-Paraffin: GAT-1/SLC6A1 Antibody (HL3475) [NBP3-48818] -

GAT-1/SLC6A1 antibody [HL3475] detects GAT-1/SLC6A1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: GAT-1/SLC6A1 stained by GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GAT-1/SLC6A1 Antibody (HL3475)

Western Blot: GAT-1/SLC6A1 Antibody (HL3475) [NBP3-48818] -

Unboiled human tissue extract was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GAT-1/SLC6A1 Antibody (HL3475)

Immunohistochemistry-Paraffin: GAT-1/SLC6A1 Antibody (HL3475) [NBP3-48818] -

GAT-1/SLC6A1 antibody [HL3475] detects GAT-1/SLC6A1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human tissues.
GAT-1/SLC6A1 stained by GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Corresponding RNA levels (RPKM) in the tissues are based on NCBI database.
GAT-1/SLC6A1 Antibody (HL3475)

Western Blot: GAT-1/SLC6A1 Antibody (HL3475) [NBP3-48818] -

Unboiled various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GAT-1/SLC6A1 Antibody (HL3475)

Western Blot: GAT-1/SLC6A1 Antibody (HL3475) [NBP3-48818] -

Boiled and unboiled rat tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GAT-1/SLC6A1 Antibody (HL3475)

Immunohistochemistry-Frozen: GAT-1/SLC6A1 Antibody (HL3475) [NBP3-48818] -

GAT-1/SLC6A1 antibody [HL3475] detects GAT-1/SLC6A1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse eye.
Green: GAT-1/SLC6A1 stained by GAT-1/SLC6A1 antibody [HL3475] (NBP3-48818) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for GAT-1/SLC6A1 Antibody (HL3475)

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAT-1/SLC6A1

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl negative channel associated with the GABAA receptor (GABAA-R) subtype. GABA plasma membrane transporters (GATs) influence synaptic neurotransmission by high-affinity uptake and release of GABA. To date, four distinct GABA transporters have been identified: GAT-1, GAT-2, GAT-3, and BGT-1. GAT-1, the most abundant of the transporters, is found predominantly in neurons, but also in some specialized glia (Minelli et al., 1995). GAT-1 is thought to play a key role in epileptogenesis (Zhao et al. 2003).

Long Name

GABA Transporter 1

Alternate Names

GABATHG, GABATR, GAT1, SLC6A1

Gene Symbol

SLC6A1

Additional GAT-1/SLC6A1 Products

Product Documents for GAT-1/SLC6A1 Antibody (HL3475)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GAT-1/SLC6A1 Antibody (HL3475)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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