GATM Antibody (OTI1E3)

Novus Biologicals | Catalog # NBP2-00984

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1E3
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Product Specifications

Immunogen

Full length human recombinant protein of human GATM(NP_001473) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

44.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GATM Antibody (OTI1E3)

Western Blot: GATM Antibody (OTI1E3) [NBP2-00984]

Western Blot: GATM Antibody (OTI1E3) [NBP2-00984]

Western Blot: GATM Antibody (1E3) [NBP2-00984] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATM.
Immunocytochemistry/Immunofluorescence: GATM Antibody (1E3) [NBP2-00984] Staining of COS7 cells transiently transfected by pCMV6-ENTRY GATM.
Immunohistochemistry-Paraffin: GATM Antibody (OTI1E3) [NBP2-00984]

Immunohistochemistry-Paraffin: GATM Antibody (OTI1E3) [NBP2-00984]

Immunohistochemistry-Paraffin: GATM Antibody (1E3) [NBP2-00984] - Staining of paraffin-embedded Human pancreas tissue using anti-GATM mouse monoclonal antibody.
Flow Cytometry: GATM Antibody (OTI1E3) [NBP2-00984]

Flow Cytometry: GATM Antibody (OTI1E3) [NBP2-00984]

Flow Cytometry: GATM Antibody (1E3) [NBP2-00984] - Analysis of Jurkat cells, using anti-GATM antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Immunohistochemistry-Paraffin: GATM Antibody (OTI1E3) [NBP2-00984]

Immunohistochemistry-Paraffin: GATM Antibody (OTI1E3) [NBP2-00984]

Immunohistochemistry-Paraffin: GATM Antibody (1E3) [NBP2-00984] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GATM mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GATM Antibody (OTI1E3) [NBP2-00984]

Immunohistochemistry-Paraffin: GATM Antibody (OTI1E3) [NBP2-00984]

Immunohistochemistry-Paraffin: GATM Antibody (1E3) [NBP2-00984] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GATM mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GATM Antibody (OTI1E3) [NBP2-00984]

Immunohistochemistry-Paraffin: GATM Antibody (OTI1E3) [NBP2-00984]

Immunohistochemistry-Paraffin: GATM Antibody (1E3) [NBP2-00984] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GATM mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GATM Antibody (OTI1E3) [NBP2-00984]

Immunohistochemistry-Paraffin: GATM Antibody (OTI1E3) [NBP2-00984]

Immunohistochemistry-Paraffin: GATM Antibody (1E3) [NBP2-00984] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-GATM mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GATM Antibody (OTI1E3) [NBP2-00984]

Immunohistochemistry-Paraffin: GATM Antibody (OTI1E3) [NBP2-00984]

Immunohistochemistry-Paraffin: GATM Antibody (1E3) [NBP2-00984] - Staining of paraffin-embedded Human breast tissue using anti-GATM mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GATM Antibody (OTI1E3) [NBP2-00984]

Immunohistochemistry-Paraffin: GATM Antibody (OTI1E3) [NBP2-00984]

Immunohistochemistry-Paraffin: GATM Antibody (1E3) [NBP2-00984] - Staining of paraffin-embedded Human Kidney tissue using anti-GATM mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GATM Antibody (OTI1E3) [NBP2-00984]

Immunohistochemistry-Paraffin: GATM Antibody (OTI1E3) [NBP2-00984]

Immunohistochemistry-Paraffin: GATM Antibody (1E3) [NBP2-00984] - Staining of paraffin-embedded Human liver tissue using anti-GATM mouse monoclonal antibody.
Flow Cytometry: GATM Antibody (OTI1E3) [NBP2-00984]

Flow Cytometry: GATM Antibody (OTI1E3) [NBP2-00984]

Flow Cytometry: GATM Antibody (1E3) [NBP2-00984] - Analysis of Hela cells, using anti-GATM antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for GATM Antibody (OTI1E3)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.95 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GATM

This gene encodes a mitochondrial enzyme that belongs to the amidinotransferase family. This enzyme is involved in creatine biosynthesis, whereby it catalyzes the transfer of a guanido group from L-arginine to glycine, resulting in guanidinoacetic acid, the immediate precursor of creatine. Mutations in this gene cause arginine:glycine amidinotransferase deficiency, an inborn error of creatine synthesis characterized by mental retardation, language impairment, and behavioral disorders. [provided by RefSeq].

Alternate Names

AGATtransamidinase, AT, EC 2.1.4, EC 2.1.4.1, glycine amidinotransferase (L-arginine:glycine amidinotransferase), glycine amidinotransferase, mitochondrial, L-arginine:glycine amidinotransferase, Transamidinase

Entrez Gene IDs

2628 (Human)

Gene Symbol

GATM

Additional GATM Products

Product Documents for GATM Antibody (OTI1E3)

Certificate of Analysis

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Product Specific Notices for GATM Antibody (OTI1E3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GATM Antibody (OTI1E3)

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Protocols

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