GBE1 Antibody (OTI1D11) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70810

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1D11

Format

Azide and BSA Free
View all GBE1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human GBE1 (NP_000149) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

80.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GBE1 Antibody (OTI1D11) - Azide and BSA Free

Western Blot: GBE1 Antibody (OTI1D11)Azide and BSA Free [NBP2-70810]

Western Blot: GBE1 Antibody (OTI1D11)Azide and BSA Free [NBP2-70810]

Western Blot: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1.
Immunocytochemistry/ Immunofluorescence: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810]

Immunocytochemistry/ Immunofluorescence: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810]

Immunocytochemistry/Immunofluorescence: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - Staining of HepG2 cells using anti-GBE1 mouse monoclonal antibody.
Immunohistochemistry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810]

Immunohistochemistry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810]

Immunohistochemistry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - Staining of paraffin-embedded Human Ovary tissue using anti-GBE1 mouse monoclonal antibody.
Flow Cytometry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810]

Flow Cytometry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810]

Flow Cytometry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GBE1.
Western Blot: GBE1 Antibody (OTI1D11)Azide and BSA Free [NBP2-70810]

Western Blot: GBE1 Antibody (OTI1D11)Azide and BSA Free [NBP2-70810]

Western Blot: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-GBE1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810]

Immunohistochemistry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810]

Immunohistochemistry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - Staining of paraffin-embedded Human colon tissue using anti-GBE1 mouse monoclonal antibody.
Immunohistochemistry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810]

Immunohistochemistry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810]

Immunohistochemistry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - Staining of paraffin-embedded Human endometrium tissue using anti-GBE1 mouse monoclonal antibody.
Flow Cytometry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810]

Flow Cytometry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810]

Flow Cytometry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-GBE1 antibody, and then analyzed by flow cytometry.
Immunoprecipitation: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810]

Immunoprecipitation: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810]

Immunoprecipitation: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - (Negative control: IP without adding anti-GBE1 antibody.). For each experiment, 500ul of DDK tagged GBE1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GBE1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Applications for GBE1 Antibody (OTI1D11) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50-100

Immunohistochemistry

1:50

Immunoprecipitation

2ug/500ul

Western Blot

1:500-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GBE1

GBE1 is encoded by this gene is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease).

Alternate Names

1,4-alpha-glucan-branching enzyme, amylo-(1,4 to 1,6) transglucosidase, amylo-(1,4 to 1,6) transglycosylase, Brancher enzyme, EC 2.4.1.18, GBE, glucan (1,4-alpha), branching enzyme 1, glycogen branching enzyme, Glycogen-branching enzyme

Gene Symbol

GBE1

Additional GBE1 Products

Product Documents for GBE1 Antibody (OTI1D11) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GBE1 Antibody (OTI1D11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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