Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all GBP1 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 500-550 of human GBP1 was used as the immunogen for this antibody.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GBP1 Antibody

Western Blot: GBP1 Antibody [NBP2-03972]

Western Blot: GBP1 Antibody [NBP2-03972]

Western Blot: GBP1 Antibody [NBP2-03972] - Analysis of GBP1 using GBP1 polyclonal antibody. Human HCT-116 lysate in the absence (1) and presence (2) of immunizing peptide and mouse RAW cell lysate (3) probed with GBP1 antibody at 3 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: GBP1 Antibody [NBP2-03972]

Immunohistochemistry-Paraffin: GBP1 Antibody [NBP2-03972]

Immunohistochemistry-Paraffin: GBP1 Antibody [NBP2-03972] - Analysis of GBP1 using GBP1 polyclonal antibody. Human rectum tissue probed with GBP1 antibody at 5 ug/ml.

Applications for GBP1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

2-4 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GBP1

GBP1 or guanlyate binding protein 1 is a 68 kDa GTPase and member of the GTPase1 family of molecules (Kunzelman, 2006). GBP1 expression is induced by Interferon g as well as other inflammatory cytokines including IL-1a, IL-1b and TNF-a (Naschberger, 2004). Induction of GBP1 expression by poly I:C, a prototypical TLR3 ligand, as well as GBP1 expression dependency on NF-kB and IFNa response elements implicate TLR pathway involvement (Naschberger, 2004). Inversely proportional expression of GBP1 and MMP-1 as well as other related molecules to adhesion and migration show a clear functional role of GBP1 in suppressing angiogenesis and migration especially of endothelial cells (Guenzi, 2003). As a regulatory molecule it is centrally involved in signal transduction of inflammatory and cell migration networks.

Alternate Names

GBP-1, GTP-binding protein 1, Guanine nucleotide-binding protein 1, guanylate binding protein 1, interferon-inducible, guanylate binding protein 1, interferon-inducible, 67kDa, HuGBP-1, interferon-induced guanylate-binding protein 1

Gene Symbol

GBP1

UniProt

NP_002044.2 (Human)

Additional GBP1 Products

Product Documents for GBP1 Antibody

Certificate of Analysis

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Product Specific Notices for GBP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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