GBX2 Antibody

Novus Biologicals | Catalog # NBP2-43617

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all GBX2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GBX2. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GBX2 Antibody

Western Blot: GBX2 Antibody [NBP2-43617]

Western Blot: GBX2 Antibody [NBP2-43617]

Western Blot: GBX2 Antibody [NBP2-43617] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody diluted at 1:1000.
Western Blot: GBX2 Antibody [NBP2-43617]

Western Blot: GBX2 Antibody [NBP2-43617]

Western Blot: GBX2 Antibody [NBP2-43617] - Analysis of IMR32 whole cell extracts and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody diluted by 1:1000.
Western Blot: GBX2 Antibody [NBP2-43617]

Western Blot: GBX2 Antibody [NBP2-43617]

Western Blot: GBX2 Antibody [NBP2-43617] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody diluted at 1:1000.
Western Blot: GBX2 Antibody [NBP2-43617]

Western Blot: GBX2 Antibody [NBP2-43617]

Western Blot: GBX2 Antibody [NBP2-43617] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody diluted at 1:1000.
GBX2 Antibody

Western Blot: GBX2 Antibody [NBP2-43617] -

Western Blot: GBX2 Antibody [NBP2-43617] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody (NBP2-43617) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GBX2 Antibody

Western Blot: GBX2 Antibody [NBP2-43617] -

Western Blot: GBX2 Antibody [NBP2-43617] - GBX2 antibody detects GBX2 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody (NBP2-43617) diluted by 1:1000.
GBX2 Antibody

Immunocytochemistry/ Immunofluorescence: GBX2 Antibody [NBP2-43617] -

GBX2 antibody detects GBX2 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GBX2 protein stained by GBX2 antibody (NBP2-43617) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:50.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for GBX2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GBX2

GBX2 (Gastrulation brain homeobox 2) is a homeobox protein which is involved in enbryonic cell pluripotency and differentiation. GBX2 is expressed in all three embryonic germ layers of the late gastrula/early neurula. Gbx2 is overexpressed in a panel of human prostatic cancer cell lines (TSU-pr1, PC3, DU145, LNCaP).

Long Name

Gastrulation Brain Homeobox 2

Alternate Names

Gastrulation and brain-specific homeobox protein 2, gastrulation brain homeo box 2, gastrulation brain homeobox 2, homeobox protein GBX-2

Gene Symbol

GBX2

Additional GBX2 Products

Product Documents for GBX2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GBX2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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