GCLC Antibody (0X1H3)

Novus Biologicals | Catalog # NBP3-16422

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0X1H3 expressed in HEK293
View all GCLC Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 538-637 of human GCLC (P48506). LNSYLENMEVDVDTRCSILNYLKLIKKRASGELMTVARWMREFIANHPDYKQDSVITDEMNYSLILKCNQIANELCECPELLGSAFRKVKYSGSKTDSSN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GCLC Antibody (0X1H3)

Western Blot: GCLC Antibody (0X1H3) [NBP3-16422]

Western Blot: GCLC Antibody (0X1H3) [NBP3-16422]

Western Blot: GCLC Antibody (0X1H3) [NBP3-16422] - Analysis of extracts of Rat kidney, using GCLC Rabbit mAb (NBP3-16422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: GCLC Antibody (0X1H3) [NBP3-16422]

Immunohistochemistry-Paraffin: GCLC Antibody (0X1H3) [NBP3-16422]

Immunohistochemistry-Paraffin: GCLC Antibody (0X1H3) [NBP3-16422] - Rat kidney using GCLC Rabbit mAb (NBP3-16422) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western Blot: GCLC Antibody (0X1H3) [NBP3-16422]

Western Blot: GCLC Antibody (0X1H3) [NBP3-16422]

Western Blot: GCLC Antibody (0X1H3) [NBP3-16422] - Analysis of extracts of various cell lines, using GCLC Rabbit mAb (NBP3-16422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Applications for GCLC Antibody (0X1H3)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GCLC

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

Long Name

Glutamate--cysteine ligase catalytic subunit

Alternate Names

EC 6.3.2.2, Gamma-ECS, GCS heavy chain, GLCL, GLCLC

Gene Symbol

GCLC

Additional GCLC Products

Product Documents for GCLC Antibody (0X1H3)

Certificate of Analysis

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Product Specific Notices for GCLC Antibody (0X1H3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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