GCLC Antibody (OTI1A3) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70830

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A3

Format

Azide and BSA Free
View all GCLC Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human GCLC(NP_001489) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

72.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GCLC Antibody (OTI1A3) - Azide and BSA Free

Western Blot: GCLC Antibody (OTI1A3)Azide and BSA Free [NBP2-70830]

Western Blot: GCLC Antibody (OTI1A3)Azide and BSA Free [NBP2-70830]

Western Blot: GCLC Antibody (OTI1A3) - Azide and BSA Free [NBP2-70830] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCLC.
Immunohistochemistry: GCLC Antibody (OTI1A3) - Azide and BSA Free [NBP2-70830]

Immunohistochemistry: GCLC Antibody (OTI1A3) - Azide and BSA Free [NBP2-70830]

Immunohistochemistry: GCLC Antibody (OTI1A3) - Azide and BSA Free [NBP2-70830] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Western Blot: GCLC Antibody (OTI1A3)Azide and BSA Free [NBP2-70830]

Western Blot: GCLC Antibody (OTI1A3)Azide and BSA Free [NBP2-70830]

Western Blot: GCLC Antibody (OTI1A3) - Azide and BSA Free [NBP2-70830] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: GCLC Antibody (OTI1A3) - Azide and BSA Free [NBP2-70830]

Immunohistochemistry: GCLC Antibody (OTI1A3) - Azide and BSA Free [NBP2-70830]

Immunohistochemistry: GCLC Antibody (OTI1A3) - Azide and BSA Free [NBP2-70830] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: GCLC Antibody (OTI1A3) - Azide and BSA Free [NBP2-70830]

Immunohistochemistry: GCLC Antibody (OTI1A3) - Azide and BSA Free [NBP2-70830]

Immunohistochemistry: GCLC Antibody (OTI1A3) - Azide and BSA Free [NBP2-70830] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for GCLC Antibody (OTI1A3) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:200-1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GCLC

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

Long Name

Glutamate--cysteine ligase catalytic subunit

Alternate Names

EC 6.3.2.2, Gamma-ECS, GCS heavy chain, GLCL, GLCLC

Gene Symbol

GCLC

Additional GCLC Products

Product Documents for GCLC Antibody (OTI1A3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GCLC Antibody (OTI1A3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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