GCLM Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16624

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (96%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GCLM Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GCLM. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GCLM Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GCLM Antibody [NBP2-16624]

Immunocytochemistry/ Immunofluorescence: GCLM Antibody [NBP2-16624]

Immunocytochemistry/Immunofluorescence: GCLM Antibody [NBP2-16624] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Western Blot: GCLM Antibody [NBP2-16624]

Western Blot: GCLM Antibody [NBP2-16624]

Western Blot: GCLM Antibody [NBP2-16624] - Sample (50 ug of whole cell lysate) A: rat liver, 12% SDS PAGE gel, diluted at 1:1000.
Western Blot: GCLM Antibody [NBP2-16624]

Western Blot: GCLM Antibody [NBP2-16624]

Western Blot: GCLM Antibody [NBP2-16624] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GCLM Antibody - BSA Free

Western Blot: GCLM Antibody [NBP2-16624] -

Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody (NBP2-16624) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GCLM Antibody - BSA Free

Western Blot: GCLM Antibody [NBP2-16624] -

Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody (NBP2-16624) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GCLM Antibody - BSA Free

Western Blot: GCLM Antibody [NBP2-16624] -

Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody (NBP2-16624) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for GCLM Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GCLM

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq]

Alternate Names

Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, GCS light chain, GLCLRglutamate--cysteine ligase regulatory subunit, glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory(30.8kD), Glutamate--cysteine ligase modifier subunit, glutamate-cysteine ligase regulatory protein, glutamate-cysteine ligase, modifier subunit, GSC light chain

Gene Symbol

GCLM

UniProt

P48507 (Human)

Additional GCLM Products

Product Documents for GCLM Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GCLM Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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