GCP4 Antibody (812) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-43586
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Chimpanzee (100%), Mouse (97%), Rabbit (97%), Xenopus (90%), Zebrafish (90%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 812
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human GCP4. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (90%).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GCP4 Antibody (812) - Azide and BSA Free
Western Blot: GCP4 Antibody (812) [NBP2-43586]
Western Blot: GCP4 Antibody (812) [NBP2-43586] - Analysis of A. 30 ug HeLa whole cell lysate/extract 10 % SDS-PAGE GCP4 antibody [812] dilution: 1:1000Immunocytochemistry/ Immunofluorescence: GCP4 Antibody (812) [NBP2-43586]
Immunocytochemistry/Immunofluorescence: GCP4 Antibody (812) [NBP2-43586] - HeLa cells were fixed in 4% paraformaldehyde at room temperature. Green: GCP4 protein stained by GCP4 antibody [812] diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 um.Applications for GCP4 Antibody (812) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: GCP4
Alternate Names
76PhGCP4, FLJ14797, gamma tubulin ring complex protein (76p gene), Gamma-ring complex protein 76 kDa, gamma-tubulin complex component 4, GCP-4, GCP4hGrip76, h76p, tubulin, gamma complex associated protein 4
Gene Symbol
TUBGCP4
Additional GCP4 Products
Product Documents for GCP4 Antibody (812) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GCP4 Antibody (812) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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