Gelsolin/GSN Antibody (3G5) - Azide and BSA Free

Novus Biologicals | Catalog # H00002934-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3G5

Format

Azide and BSA Free
View all Gelsolin/GSN Primary Antibodies »

Product Specifications

Immunogen

GSN (AAH26033, 673 a.a. ~ 782 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SNKIGRFVIEEVPGELMQEDLATDDVMLLDTWDQVFVWVGKDSQEEEKTEALTSAKRYIETDPANRDRRTPITVVKQGFEPPSFVGWFLGWDDDYWSVDPLDRAMAELAA

Specificity

GSN - gelsolin (amyloidosis, Finnish type)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Gelsolin/GSN Antibody (3G5) - Azide and BSA Free

Western Blot: Gelsolin/GSN Antibody (3G5) [H00002934-M01]

Western Blot: Gelsolin/GSN Antibody (3G5) [H00002934-M01]

Western Blot: Gelsolin/GSN Antibody (3G5) [H00002934-M01] - Analysis of GSN expression in transfected 293T cell line by GSN monoclonal antibody (M01), clone 3G5.Lane 1: GSN transfected lysate(85.7 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (3G5) [H00002934-M01]

Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (3G5) [H00002934-M01]

Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (3G5) [H00002934-M01] - Analysis of monoclonal antibody to GSN on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1 ug/ml.
Western Blot: Gelsolin/GSN Antibody (3G5) [H00002934-M01]

Western Blot: Gelsolin/GSN Antibody (3G5) [H00002934-M01]

Western Blot: Gelsolin/GSN Antibody (3G5) [H00002934-M01] - GSN monoclonal antibody (M01), clone 3G5 Analysis of GSN expression in HeLa.
Immunoprecipitation: Gelsolin/GSN Antibody (3G5) [H00002934-M01]

Immunoprecipitation: Gelsolin/GSN Antibody (3G5) [H00002934-M01]

Immunoprecipitation: Gelsolin/GSN Antibody (3G5) [H00002934-M01] - Analysis of GSN transfected lysate using anti-GSN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GSN MaxPab rabbit polyclonal antibody.
Sandwich ELISA: Gelsolin/GSN Antibody (3G5) [H00002934-M01] - Detection limit for recombinant GST tagged GSN is approximately 0.03ng/ml as a capture antibody.

Applications for Gelsolin/GSN Antibody (3G5) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Gelsolin/GSN

The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene.

Alternate Names

ADF, AGEL, Brevin, GSN

Entrez Gene IDs

2934 (Human)

Gene Symbol

GSN

OMIM

105120 (Human)

UniProt

AAH26033 (Human)

Additional Gelsolin/GSN Products

Product Documents for Gelsolin/GSN Antibody (3G5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Gelsolin/GSN Antibody (3G5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Citations for Gelsolin/GSN Antibody (3G5) - Azide and BSA Free

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Protocols

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FAQs

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